Tissue microarray analysis of interleukin-20 expression
Tissue microarray analysis of interleukin-20 expression
复制标题
DOI：
10.1016/j.cyto.2006.07.006
复制
发表时间：
2006-07-01
期刊：
影响因子：
3.8
通讯作者：
Chang, Ming-Shi
中科院分区：
文献类型：
--
作者：
Hsing, Chung-Hsi;Ho, Chung-Liang;Chang, Ming-Shi
白介素20是新近发现的白介素10家族中的一种细胞因子，对其生物学功能和临床意义的了解尚不完全。我们的目的是确定IL-20在健康组织和肿瘤组织中的表达分布，并描述表达IL-20的细胞类型，因为这些信息将显著影响对其病理生理作用的探索。我们使用组织芯片技术和使用抗IL-20单抗的免疫组织化学方法检测了36例非肿瘤组织和14例肿瘤组织中IL-20的表达。IL-20蛋白在30种非肿瘤性组织类型和5种主要细胞类型：上皮细胞、肌上皮细胞、内皮细胞、巨噬细胞和骨骼肌细胞中呈阳性表达。我们还发现，几种类型的肿瘤细胞IL-20染色呈阳性，特别是在皮肤、舌、食道和肺的鳞状细胞癌中。我们的数据为进一步研究IL-20在人类中的生物学功能和临床意义提供了有价值的参考。(C)2006爱思唯尔有限公司。保留所有权利。
Knowledge about the biological functions and clinical implications of interleukin (IL)-20, a recently discovered cytokine in the IL-10 family, is still incomplete. Our aim was to determine the distribution of IL-20 expression and to delineate the cell types that express IL-20 in healthy and neoplastic tissue, because this information will significantly affect the exploration of its pathophysiological roles. We used tissue microarray technology and an immunohistochemical survey using an anti-IL-20 monoclonal antibody to examine IL-20 expression in 36 non-neoplastic and 14 neoplastic tissues. IL-20 protein was positively stained in 30 non-neoplastic tissue types and five major cell types: epithelial cells, myoepithelial cells, endothelial cells, macrophages, and skeletal muscle cells. We also found that several types of tumor cells stained positive for IL-20, especially in squamous cell carcinoma of the skin, tongue, esophagus, and lung. Our data provide valuable references for further investigation of the biological functions and clinical implications of IL-20 in humans. (c) 2006 Elsevier Ltd. All rights reserved.