Downregulation of EphA2 expression suppresses the growth and metastasis in squamous-cell carcinoma of the head and neck in vitro and in vivo
Downregulation of EphA2 expression suppresses the growth and metastasis in squamous-cell carcinoma of the head and neck in vitro and in vivo
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DOI：
10.1007/s00432-011-1087-9
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发表时间：
2012-02-01
影响因子：
3.6
通讯作者：
Tian, Yongquan
中科院分区：
文献类型：
--
作者：
Liu, Yong;Yu, Changyun;Tian, Yongquan
目的我们的前期研究表明EphA 2过表达与头颈部鳞状细胞癌（SCCHN）患者的侵袭行为和不良预后显著相关。然而，EphA 2在SCCHN肿瘤发生和颈淋巴结转移中的作用尚未在体内阐明。体外CCK-8测定、Xu细胞分选分析、侵袭和迁移测定。结果EphA 2的抑制可显著抑制肿瘤细胞的增殖、迁移、增殖和转移，结论EphA 2在SCCHN细胞的生长和转移中起重要作用，可能与EphA 2在SCCHN细胞的增殖和转移中起重要作用有关。是一个有前途的治疗靶点，以防止SCCHN的进展。
Purpose Our previous study has revealed that EphA2 overexpression is significantly associated with aggressive behavior and poor prognosis in patients with squamous-cell carcinoma of the head and neck (SCCHN). However, the function of EphA2 in tumorigenesis and cervical lymph node metastasis of SCCHN has never been elucidated in vivo.Methods EphA2 was knocked down in SCCHN cell lines. CCK-8 assays, Xuorescence-activated cell sorting analysis, invasion and migration assays were performed in vitro. In vivo tumorigenicity assays were performed, and the impact on cervical lymph node metastasis was evaluated.Results The present investigation demonstrated that suppression of EphA2 resulted in a significant inhibition of proliferation, migration, invasion of SCCHN cells in vitro and markedly diminished their tumorigenicity and lymph node metastasis in vivo.Conclusions These results suggest that EphA2 plays a critical role in SCCHN growth and metastasis and may be a promising therapeutic target to prevent the progression of SCCHN.