Exploring the nano-fungicidal efficacy of green synthesized magnesium oxide nanoparticles (MgO NPs) on the development, physiology, and infection of carrot (Daucus carota L.) with Alternaria leaf blight (ALB): Molecular docking
Exploring the nano-fungicidal efficacy of green synthesized magnesium oxide nanoparticles (MgO NPs) on the development, physiology, and infection of carrot (Daucus carota L.) with Alternaria leaf blight (ALB): Molecular docking
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DOI：
10.1016/j.jia.2023.02.034
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发表时间：
2023-10-09
影响因子：
4.8
通讯作者：
Alam，Mahboob
中科院分区：
文献类型：
--
作者：
Ahamad，Lukman;Khan，Azmat A. L.;Alam，Mahboob
本研究以柠檬果实提取物为原料，制备了绿色氧化镁纳米颗粒（MgO NPs），并研究了其对胡萝卜黑斑病（Alternaria dauci）的抑制作用。在温室条件下。采用扫描电镜、透射电镜和紫外可见光谱对纳米颗粒进行了表征。使用选区电子衍射（SAED）确定MgONP的结晶性质。当暴露于50和100 mg L− 1浓度时，MgO NPs触发了对胡萝卜素的显著抗真菌活性，但在体外条件下，在100 mg L− 1浓度下观察到更高的抗真菌潜力。在真菌接种的植物中，胡萝卜的生长，光合色素显着下降，酚，脯氨酸含量和防御相关酶的增加被视为超过对照（蒸馏水）。然而，叶面施用50和100 mg L− 1的MgO NPs显著改善了植物生长、光合色素、酚和脯氨酸含量以及有和没有A.dauci感染的胡萝卜的防御酶活性。100 mg L− 1的MgO NPs处理的胡萝卜在受到A.接种对照。当喷洒MgO NPs时，接种了胡萝卜的植物的叶枯病指数和疾病严重程度百分比也降低。利用分子对接技术研究了链格孢菌蛋白与纳米颗粒的非键相互作用。
In this research, green synthesized magnesium oxide nanoparticles (MgO NPs) from lemon fruit extracts and their fungicidal potential was evaluated againstAlternaria dauciinfection on carrot (Daucus carotaL.) under greenhouse conditions. The scanning and transmission electron microscopy (SEM and TEM) and ultra-violet (UV) visible spectroscopy were used to validate and characterize MgO NPs. The crystalline nature of MgONPs was determined using selected area electron diffraction (SAED). MgO NPs triggered substantial antifungal activity againstA.dauciwhen exposed to 50 and 100 mg L−1concentrations but the higher antifungal potential was noticed in 100 mg L−1underin-vitroconditions. In fungal inoculated plants, a marked decrease in growth, photosynthetic pigments, and an increase in phenol, proline contents, and defense-related enzymes of carrot were seen over control (distilled water). However, foliar application of MgO NPs at 50 and 100 mg L−1resulted in significant improvement of plant growth, photosynthetic pigments, phenol and proline contents, and defense enzymes activity of carrots with and withoutA.dauciinfection. Spraying of MgO NPs at 100 mg L−1had more plant length (17.11%), shoot dry weight (34.38%), plant fresh weight (20.46%), and root dry weight (49.09%) in carrots when challenged withA. dauciover inoculated control. The leaf blight indices and percent disease severity were also reduced inA.dauciinoculated plants when sprayed with MgO NPs. The non-bonding interactions ofAlternariagenus protein with nanoparticles were studied using molecular docking.