Intrathymic T cell differentiation in radiation bone marrow chimeras and its role in T cell emigration to the spleen. An immunohistochemical study.
Intrathymic T cell differentiation in radiation bone marrow chimeras and its role in T cell emigration to the spleen. An immunohistochemical study.
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放射骨髓嵌合体中胸腺内 T 细胞的分化及其在 T 细胞迁移至脾脏中的作用。
DOI：
10.4049/jimmunol.134.6.3615
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发表时间：
1985
影响因子：
4.4
通讯作者：
M. Utsuyama
中科院分区：
文献类型：
--
作者：
K. Hirokawa;T. Sado;S. Kubo;H. Kamisaku;K. Hitomi;M. Utsuyama
用B10-和B10对放射骨髓嵌合体胸腺和脾脏中宿主型和供体型T细胞的再生进行免疫组化研究。BR-Thy-1基因小鼠。无论供体和宿主之间的H-2相容性如何，胸腺皮层和髓质都首先用辐照源宿主的胸腺细胞重新填充，在第11至14天恢复正常的组织学外观。在Thy-1基因嵌合体中，在第7天首次识别到来自供体骨髓的胸腺细胞，在一个胸腺叶中不到100个细胞，此时胸腺在照射后累及到最小的大小。然后供体型胸腺细胞呈指数增殖，在高剂量骨髓细胞重建时增殖速度略快，到第17天达到1亿个水平，到第21天完全取代宿主源性皮质胸腺细胞。皮质胸腺细胞的替换从包膜下层零星开始。髓质胸腺细胞从宿主型向供体型的替换在皮层的替换完成后的第21天至第35天逐渐发生。在脾脏中，照射后第3天回收了约100万个存活细胞，其中约60%为宿主型T细胞，可见于白髓区。在第10天之后，由于来自再生胸腺的宿主T细胞的涌入，脾脏中的宿主T细胞逐渐增加。因此，免疫组织化学观察到宿主型T细胞在第21 ~ 28天的脾脏白髓中明显增加，而宿主型T细胞主要存在于胸腺髓质中。这些宿主型T细胞在脾脏中存活了很长时间，在治疗后长达420天。表型上，在第28天检测时，它们以Lyt-1+2+为主，但5个月后，它们约为50% Lyt-1+2+和50% Lyt-1+2-。在移植了大剂量骨髓细胞的嵌合体中，脾脏中的供体型T细胞大约在第14天开始出现，而在移植了小剂量骨髓细胞的嵌合体中，这种情况稍微延迟，大约在第28天开始出现。（摘要删节为400字）
Immunohistochemical studies were made on the regeneration of T cells of host- and donor-type in the thymus and spleen of radiation bone marrow chimeras by using B10- and B10.BR-Thy-1 congenic mice. Both the thymic cortex and the medulla were first repopulated with thymocytes of irradiated host origin, restoring the normal histologic appearance by days 11 to 14, regardless of the H-2 compatibility between the donor and the host. In Thy-1 congenic chimeras, thymocytes of donor bone marrow origin, less than 100 cells in one thymic lobe, were first recognized at day 7, when the thymus involuted to the smallest size after the irradiation. The thymocytes of donor-type then proliferated exponentially, showing a slightly faster rate when higher doses of bone marrow cells were used for reconstitution, reaching a level of 100 million by day 17 and completely replacing the cortical thymocytes of host origin by day 21. The replacement of cortical thymocytes started from the subcapsular layer in a sporadic manner. The replacement of medullary thymocytes from host- to donor-type occurred gradually between days 21 and 35, after the replacement in the cortex was completed. In the spleen, about 1 million survived cells were recovered at day 3 after the irradiation, and approximately 60% of them were shown to be host-type T cells that were observed in the white pulp areas. The host-type T cells in the spleen increased gradually after day 10, due to the influx of host-type T cells from the regenerating thymus. Thus a pronounced increase of T cells of host-type was immunohistochemically observed in the splenic white pulp between days 21 and 28, when thymocytes of host-type were present mainly in the thymic medulla. These host-type T cells were shown to persist in the spleen for a long time, as long as 420 days after the treatment. Phenotypically, they were predominantly Lyt-1+2+ when examined at day 28, but 5 mo later, they were about 50% Lyt-1+2+ and 50% Lyt-1+2-. Donor-type T cells in the spleen began to appear at about day 14 in chimeras that were transplanted with a larger dose of bone marrow cells, whereas this was slightly delayed in those grafted with a smaller dose of bone marrow cells, starting at about day 28.(ABSTRACT TRUNCATED AT 400 WORDS)