Genetic Difference among Seed Populations of Greater Amberjack Used in Aquaculture Farm of Japan
Genetic Difference among Seed Populations of Greater Amberjack Used in Aquaculture Farm of Japan
复制标题
日本水产养殖场大鰤鱼苗群体的遗传差异
DOI：
10.11233/aquaculturesci1953.48.665
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发表时间：
2000
期刊：
影响因子：
--
通讯作者：
S. Miyashita
中科院分区：
文献类型：
--
作者：
E. Nugroho;N. Taniguchi;Keitaro Kato;S. Miyashita
以日本近海采集的种子鱼和人工繁殖的种子鱼为研究对象，对其遗传变异性进行了研究。利用微卫星DNA(MS)和线粒体DNA(MtDNA)D-loop标记进行遗传变异分析。水产养殖、天然种子和人工种子每个座位的平均等位基因数分别为11.0、11.5和4.3.平均观察(和预期)杂合度分别为0.701(0.778)、0.654(0.738)和0.532(0.540)。用Taq I、Hae III、Mbo I、Rsa I和Hinf I 5种内切酶对D-loop序列进行酶切后，检测到33种mtDNA单倍型。基于这两个座位MS的NEI遗传距离显示，至少有两个遗传不同的亚群被用于水产养殖
Genetic variability of greater amberjack used as seed fish in aquaculture was examined, and compared with those of collected from coast waters of Japan and artificial propagated activity. The genetic variability was assessed using polymorphism of microsatellite DNA (MS) and the mitochondrial DNA (mtDNA) D-loop markers. The average alleles number per locus were 11.0, 11.5 and 4.3 for aquaculture, natural and artificial seed respectively, while the average observed (and expected) heterozygosities were 0.701 (0.778), 0.654 (0.738) and 0.532 (0.540). Thirty-three mtDNA haplotypes were detected following digestion of D-loop sequences with five endonucleases: Taq I, Hae III, Mbo I, Rsa I and Hinf I. Significant genetic difference was observed among seed population of greater amberjack. Nei’s genetic distances based on the two loci MS revealed at least two genetically different sub-populations of greater amberjack were used in the aquaculture
影响因子：
3.3
作者：
M. Nei;F. Tajima
通讯作者：
M. Nei;F. Tajima