Application of real-time PCR on the development of molecular markers and to evaluate critical aspects for olive oil authentication
Application of real-time PCR on the development of molecular markers and to evaluate critical aspects for olive oil authentication
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DOI：
10.1016/j.foodchem.2009.05.012
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发表时间：
2010-01-15
期刊：
影响因子：
8.8
通讯作者：
Atienza, Sergio G.
中科院分区：
文献类型：
--
作者：
Gimenez, Maria J.;Piston, Fernando;Atienza, Sergio G.
使用基于DNA的标记进行橄榄油认证变得非常重要。qRT-PCR是一种有效的工具，在调查橄榄油认证的PCR引物的效用，允许丢弃低PCR效率的引物。它还允许研究四种DNA分离方法之间的相对有效性，因此qRT-PCR将有助于进一步优化DNA提取方案。80 bp扩增子扩增的靶分子数高于200 bp扩增子扩增的靶分子数。因此，扩增子的大小应针对橄榄油认证进行优化，因为模板的数量越多，成功扩增的可能性就越大。总之，qRT-PCR是开发用于橄榄油认证的分子标记物的有用工具，它应用于优化关键参数，如扩增子大小和DNA提取方法。(C)2009爱思唯尔有限公司保留所有权利。
Olive oil authentication using DNA-based markers is becoming very important. qRT-PCR was an efficient tool in investigating the utility of PCR primers for olive oil authentication allowing to discard primers with low PCR efficiency. It also allows investigating of the relative effectiveness among four DNA isolation methods and therefore qRT-PCR would be useful for further optimisation of the DNA extraction protocols. The number of target molecules for the amplification of 80 bp amplicons was higher than that of 200 bp. Therefore the amplicon size should be optimised for olive oil authentication since the higher the number of templates the greater the probability of successful amplification. On conclusion, qRT-PCR is a useful tool in the development of molecular markers for olive oil authentication and it should be used for the optimisation of critical parameters such as the amplicon size and the DNA extraction method. (C) 2009 Elsevier Ltd. All rights reserved.