Identification and mode of action of quantitative trait loci affecting seedling height and leaf area in Eucalyptus nitens
Identification and mode of action of quantitative trait loci affecting seedling height and leaf area in Eucalyptus nitens
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DOI：
10.1007/s001220050465
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发表时间：
1997-04-01
影响因子：
5.4
通讯作者：
Moran, GF
中科院分区：
文献类型：
--
作者：
Byrne, M;Murrell, JC;Moran, GF
对光叶桉（Eucalyptusnitens）三代远交家系幼苗的高度和叶面积的基因组影响区域进行了鉴定。利用单因素方差分析定位了3个影响株高的QTL和2个影响叶面积的QTL。影响株高的3个QTL各解释10.3 ~ 14.7%的表型变异，而影响叶面积的2个QTL各解释9.8 ~ 11.6%的表型变异。与QTL连锁的完全信息标记位点的分析使QTL的作用模式得以研究。3个位点的QTL效应只与一个亲本分离，2个位点的QTL在系谱中表现为多等位基因且与双亲分离。影响叶面积的两个QTL与影响株高的两个QTL位于同一区域。分析这些地区的充分信息标记表明，这两个QTL连锁到相同的标记？但其中一个对株高和叶面积的作用大小和作用方式相似，而另一个对这两个性状的作用方式不同。这些区域可能包含两个紧密连锁的基因，或者可能涉及对高度和叶面积具有多效性效应的单个基因。具有最大效应的QTL显示多个等位基因和减少效应大小的位点内相互作用。在第二个相关的家庭中的两个QTL的评估没有显示与标记位点相关的效果，但是，这是与这些QTL的作用方式和在第二个家庭中的遗传模式一致。
Regions of the genome influencing height and leaf area in seedlings of a three-generation outbred pedigree of Eucalyptus nitens have been identified. Three QTLs affecting height and two QTLs affecting leaf area were located using single-factor analysis of variance. The three QTLs affecting height each explained between 10.3 and 14.7% of the phenotypic variance, while the two QTLs for leaf area each explained between 9.8 and 11.6% of the phenotypic variation. Analysis of fully informative marker loci linked to the QTLs enabled the mode of action of the QTLs to be investigated. For three loci the QTL effect segregated from only one parent, while for two loci the QTL showed multiple alleles and the effect segregated from both parents in the pedigree. The two QTLs affecting leaf area were located in the same regions as two of the QTLs affecting height. Analysis of these regions with fully informative markers showed that both QTLs were linked to the same markers? but one had a similar size of effects and a similar mode of action for both height and leaf area, whilst the other showed a different mode of action for the two traits. These regions may contain two closely linked genes or may involve a single gene with a pleiotrophic effect on both height and leaf area. The QTL with the greatest effect showed multiple alleles and an intra-locus interaction that reduced the size of the effect. Assessment for two of the QTLs in a second related family did not show an effect associated with the marker loci; however, this was consistent with the mode of action of these QTLs and the pattern of inheritance in the second family.