Structural characterization and osteogenic bioactivity of a sulfated polysaccharide from pacific abalone (Haliotis discus hannai Ino)
Structural characterization and osteogenic bioactivity of a sulfated polysaccharide from pacific abalone (Haliotis discus hannai Ino)
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太平洋鲍鱼（Haliotis Discus hannai Ino）硫酸化多糖的结构特征和成骨生物活性
DOI：
10.1016/j.carbpol.2017.11.022
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发表时间：
2018-02-15
影响因子：
11.2
通讯作者：
Wen, Chengrong
中科院分区：
文献类型：
--
作者：
Song, Shuang;Zhang, Bao;Wen, Chengrong
骨形态发生蛋白-2（BMP-2）已知促进骨生成。为了寻找新的佐剂来增强BMP-2的活性，本研究从皱纹盘鲍（Haliotis discus hannai Ino）性腺中提取了一种水溶性多糖AGSP，并对其结构进行了研究。通过对AGSP释放的醛二糖醛酸的分析、AGSP及其还原产物单糖组成的比较、AGSP及其衍生物的甲基化分析和NMR分析，确定AGSP的主要结构残基为-> 3）-GlcA（1-> 3）-Gal（1->具有由主要Gal和次要Glc组成的硫酸化支链，AGSP的硫酸盐含量为12.4%，相对分子量为6.6 kDa。AGSP在10 - 200 μ g/mL浓度范围内呈剂量依赖性地增强BMP-2诱导的碱性磷酸酶活性，在200 μ g/mL浓度下增强425%，表明AGSP可能是增强BMP-2成骨活性的候选佐剂。
Bone morphogenic protein-2 (BMP-2) is known to promote osteogenesis. To find novel adjuvants to enhance the activity of BMP-2, the present study investigated the structure BMP-2-induced osteogenic activity of a water-soluble polysaccharide from the gonad of pacific abalone (Haliotis discus hannai Ino) named AGSP. Through analysis of aldobiouronic acids released from AGSP, monosaccharide composition comparison of AGSP and its reduced product, and methylation analysis and NMR analysis of AGSP and its desulfated derivative, the main structure residue of AGSP was determined as -> 3)-GlcA(1 -> 3)-Gal(1 -> with sulfated branches comprised of prevelant Gal and minor Glc, and -> 4)-beta-GlcA(1 -> 2)-alpha-Man(1 -> residue was also found. AGSP possessed a sulfate content of 12.4% with a relative molecular weight of 6.6 kDa. AGSP strengthened alkaline phosphatase activity induced by BMP-2 in a dose dependent manner at 10-200 mu g/mL with 425% enhancement being observed at 200 mu g/mL, indicating AGSP could be an adjuvant candidate to enhance osteogenic activity of BMP-2.