Isolation of a putative prolyl‐tRNA synthetase (CaPRS) gene from Candida albicans
Isolation of a putative prolyl‐tRNA synthetase (CaPRS) gene from Candida albicans
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DOI：
10.1002/(sici)1097-0061(199711)13:14
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发表时间：
1997-11
期刊：
影响因子：
2.6
通讯作者：
M. Sentandreu;M. Elorza;R. Sentandreu
中科院分区：
文献类型：
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作者：
M. Sentandreu;M. Elorza;R. Sentandreu
我们从含有两个开放阅读框架(ORF)的白色念珠菌基因组文库中分离到一个4.0kb的片段。其中一个与Pro-tRNA合成酶同源，该酶催化由Pro充电的特定tRNA(Caprs)。测得575个氨基酸，代表一个66.2 kDa的多肽。FASTA检索表明，CaPRSp与酿酒酵母ORF(YER087)的同源性为54.4%，与大肠杆菌Prolyl-tRNA合成酶(COLIPRO)的同源性为43.8%。通过ProSite程序鉴定了一致的II类氨酰-tRNA合成酶序列。Caprs定位于白念珠菌基因组的R染色体上，在所有实验条件下，Caprs DNA杂交得到一个1.7kb的主要RNA转录本。提交给EMBL数据库的CaPRS序列可在登录号U86341下获得。
We have isolated a 4·0‐kb fragment from a genomic library of Candida albicans which contained two open reading frames (ORFs). One of them is homologous to a prolyl‐tRNA synthetase that catalyses the charging of a specific tRNA by proline (CaPRS). A deduced sequence of 575 amino acids representing a polypeptide of 66·2 kDa was determined. A FASTA search indicated that the CaPRSp had an overall similarity of 54·4% with the product of a Saccharomyces cerevisiae ORF (YER087) and 43·8% with the prolyl‐tRNA synthetase of Escherichia coli (COLIPRO). Consensus Class II aminoacyl‐tRNA synthetase sequences were identified by the PROSITE program. CaPRS was localized to chromosome R of the C. albicans genome and CaPRS DNA hybridized to a major RNA transcript of 1·7 kb under all conditions tested. The CaPRS sequence submitted to the EMBL data library is available under Accession Number U86341.© 1997 John Wiley & Sons, Ltd.