ANCHORAGE AND GROWTH REGULATION IN NORMAL AND VIRUS-TRANSFORMED CELLS
ANCHORAGE AND GROWTH REGULATION IN NORMAL AND VIRUS-TRANSFORMED CELLS
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DOI：
10.1002/ijc.2910030517
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发表时间：
1968-01-01
影响因子：
6.4
通讯作者：
WAXMAN, V
中科院分区：
文献类型：
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作者：
STOKER, M;ONEILL, C;WAXMAN, V
许多细胞类型在附着于刚性表面而不是悬浮液时会生长，这种现象称为“锚定依赖性”。锚定依赖性可以通过将不同尺寸的固体颗粒掺入凝胶中来研究。已经发现，集落将在500 μ长的玻璃纤维上形成，但在存在比细胞小的二氧化硅碎片的情况下不会形成。这表明悬浮培养基本身不是抑制性的，并且证实了需要足够大小的刚性表面。通过将悬浮培养物中的抑制细胞分散在甲基纤维素凝胶中，在衬有琼脂的容器中，检查悬浮培养物中的抑制细胞的状态。在该系统中，防止了聚集，并且可以定量地回收细胞。正常的细胞和转化的细胞大小增加，并且在悬浮培养的前24小时内有一部分合成DNA。正常细胞的生长和DNA合成几乎停止，而转化细胞继续生长成集落。静止的正常细胞在悬浮液中保持至少一周的进一步生长能力。当这些细胞在秋水仙素存在下附着在刚性表面时，DNA合成发生，然后进行有丝分裂。这些结果表明，悬浮细胞在有丝分裂和周期的S期结束之间被阻断。
Many cell types will grow when attached to a rigid surface but not in suspension, a phenomenon termed „anchorage dependence”︁. Anchorage dependence can be studied by incorporating solid particles of varying size into gels. It has been found that colonies will form on glass fibrils 500 μ in length, but not in the presence of silica fragments smaller than the cells. This shows that the suspending medium is not itself inhibitory, and confirms the requirement for a rigid surface of adequate size.The state of inhibited cells in suspension culture was examined by dispersing them in a methyl cellulose gel, in vessels lined with agar. In this system aggregation is prevented and the cells may be recovered quantitatively. Normal, as well as transformed, cells increase in size, and a proportion synthetize DNA during the first 24 hours in suspension culture. Growth and DNA synthesis in normal cells then virtually cease, while transformed cells continue to grow into colonies. The stationary normal cells remain competent for further growth for at least a week in suspension. When such cells are allowed to attach to a rigid surface in the presence of colchicine, DNA synthesis occurs and is followed by mitosis. These results indicate that suspended cells are blocked between mitosis and the end of the S phase of the cycle.