27-hydroxycholesterol induces the transition of MCF7 cells into a mesenchymal phenotype.
27-hydroxycholesterol induces the transition of MCF7 cells into a mesenchymal phenotype.
复制标题
DOI：
10.3892/or.2011.1284
复制
发表时间：
2011-08
期刊：
影响因子：
4.2
通讯作者：
C. Torres;M. Ramírez;P. Cruz;M. J. Epuñán;L. Valladares;W. Sierralta
中科院分区：
文献类型：
--
作者：
C. Torres;M. Ramírez;P. Cruz;M. J. Epuñán;L. Valladares;W. Sierralta
在暴露于 2 µM 27-羟基胆固醇 (27OHC) 超过 48 小时的 MCF7 细胞中，观察到 E-钙粘蛋白和 β-连环蛋白表达的减少，与粘附连接复合物的损失平行，表明上皮-间质转化 (EMT)。从培养基中除去 27OHC 后，暴露于氧甾醇 72 小时而释放的细胞生长为松散堆积的细胞群。在这些细胞中，观察到细胞质中E-钙粘蛋白和β-连环蛋白的积累以及质膜中表皮生长因子受体2（EGFR2/neu）的延长表达，表明获得的表型与这种酪氨酸激酶生长因子受体的表达有关。这里提出的结果是基于 27OHC、高胆固醇血症、巨噬细胞浸润和难治性 ERα+ 乳腺癌发病率之间所声称的关系进行讨论的。
A decrease in the expression of E-cadherin and β-catenin, paralleling the loss of adherens junction complex, was observed in MCF7 cells exposed for longer than 48 h to 2 µM 27-hydroxycholesterol (27OHC), indicating an epithelial-mesenchymal transition (EMT). Upon removal of 27OHC from the culture medium, the cells released by the exposure of 72 h to the oxysterol grew as loosely packed cell groups. In these cells, accumulation of E-cadherin and β-catenin in the cytoplasm and the prolonged expression of epidermal growth factor receptor 2 (EGFR2/neu) in the plasma membrane were observed, suggesting that the acquired phenotype was related to the expression of this tyrosine kinase-growth factor receptor. The results presented here are discussed on the basis of the claimed relationship between 27OHC, hypercholesterolemia, macrophage infiltration and therapy-resistant ERα+ breast cancer incidence.