Effect of histamine antagonists and agonists on IgE production in mice.
Effect of histamine antagonists and agonists on IgE production in mice.
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组胺拮抗剂和激动剂对小鼠 IgE 产生的影响。
DOI：
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发表时间：
1992
期刊：
影响因子：
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通讯作者：
Y. Umegae
中科院分区：
文献类型：
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作者：
T. Nakazawa;Y. Umegae
本研究探讨了组胺及其受体拮抗剂和激动剂对小鼠IgE抗体产生的影响。BALB/c小鼠腹腔内免疫1毫克明矾加30微克的Ag 90，日本职业性哮喘的抗原。在免疫前给予组胺受体拮抗剂，同时注射组胺。免疫后14天流血的，获得抗Ag 90 IgE抗体。通过Sprague-Dawley大鼠被动皮肤过敏反应测定抗血清滴度。仅用组胺治疗不影响特异性IgE抗体水平。给药H1拮抗剂或H2激动剂显著抑制IgE产生。相反，用H2拮抗剂或H1激动剂处理增强IgE抗体产生。注射H1 + H2拮抗剂对抗体产生没有影响。这些结果表明，组胺通过H2受体抑制特异性IgE的产生，并通过H1受体在系统的诱导阶段增强它。
This study was undertaken to investigate the effect of histamine, its receptor antagonists and agonists on IgE antibody production in mice. BALB/c mice were immunized intraperitoneally with 1 mg alum plus 30 micrograms of Ag90, the antigen of Japanese occupational asthma. Histamine receptor antagonists were administered before the immunization and simultaneous injection of histamine. The mice were bled 14 days after immunization and anti-Ag90 IgE antibody was obtained. Titers of the antisera were measured by passive cutaneous anaphylaxis reaction in Sprague-Dawley rats. Treatment with histamine only did not affect the level of specific IgE antibody. Administration of H1 antagonist or H2 agonist suppressed IgE production significantly. In contrast, treatment with H2 antagonist or H1 agonist augmented the IgE antibody production. Injection of H1 + H2 antagonists had no effect on the antibody production. These results suggest that histamine suppressed specific IgE production via H2 receptors and enhanced it through H1 receptors in the induction phase of the system.