Visceral adipose tissue zinc finger protein 36 mRNA levels are correlated with insulin, insulin resistance index, and adiponectinemia in women
Visceral adipose tissue zinc finger protein 36 mRNA levels are correlated with insulin, insulin resistance index, and adiponectinemia in women
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DOI：
10.1530/eje-07-0073
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发表时间：
2007-10-01
影响因子：
5.8
通讯作者：
Tchernof, Andre
中科院分区：
文献类型：
--
作者：
Bouchard, Luigi;Vohl, Marie-Claude;Tchernof, Andre
简介：脂肪组织现在被认为是一种内分泌器官，分泌许多可能参与代谢综合征病理生理学的分子和蛋白质。最近，我们已经确定了网膜脂肪组织的转录组，导致了肥胖相关代谢并发症的新候选基因的鉴定，锌指蛋白36（ZFP 36），已知其下调肿瘤坏死因子-α目的：本研究的目的是进一步研究ZFP 36基因表达水平、肥胖相关表型和脂肪因子之间的关系。腹部皮下和网膜脂肪组织样本来自46名接受择期妇科手术的妇女。通过定量实时PCR评估脂肪组织ZFP 36 mRNA丰度。通过双能X线吸收法和计算机断层扫描测量体脂积累和分布。空腹血糖、胰岛素、血脂、循环TNF-α、白细胞介素-6（IL-6）、脂联素和血清白细胞介素-6（IL-6）水平也进行了测定。ZFP 36 mRNA水平和所测试的任何表型。然而，尽管大网膜ZFP 36 mRNA水平与体脂和血脂指标无关，但与空腹胰岛素水平（R = -0.31; P = 0.05）、胰岛素抵抗指数（HOMA-IR; R = -0.31; P = 0.05）和葡萄糖后2小时胰岛素血症（R = -0.32：P = 0.05）呈负相关。大网膜ZFP 36 mRNA丰度与脂联素血症呈正相关（R = 0.35; P=0.03），但与循环TNF-α、IL-6和TNF-α浓度无关。脂肪，可以提供部分保护，防止胰岛素抵抗和糖尿病的发展。
Introduction: Adipose tissue is now recognized as an endocrine organ and secretes numerous molecules and proteins potentially involved in the physiopathology of the metabolic syndrome. Recently, we have determined the transcriptome of omental adipose tissue, leading to the identification of a new candidate gene for obesity-related metabolic complications, zinc finger protein 36 (ZFP36), which is known to down-regulate tumor necrosis factor-alpha (TNF-alpha) expression.Objective: The objective of this study was to further examine the relationship between ZFP36 gene expression levels, obesity-related phenotypes, and adipokines.Methods: Abdominal subcutaneous and omental adipose tissue samples were obtained from 46 women undergoing elective gynecological surgery. Adipose tissue ZFP36 mRNA abundance was assessed by quantitative real-time PCR. Body fat accumulation and distribution were measured by dual X-ray absorptiometry and computed tomography. Fasting blood levels of glucose, insulin, and lipids, and circulating TNF-alpha, interleukin-6 (IL-6), resistin, and adiponectin were also measured.Results: No correlation was observed between s.c. ZFP36 mRNA levels and any of the phenotypes tested. However, although omental ZFP36 mRNA levels were not correlated with measures of body fatness and lipid profile, they were negatively correlated with fasting insulin levels (R = -0.31; P = 0.05), the insulin resistance index (HOMA-IR; R = -0.31; P = 0.05), and 2-h post-glucose insulinemia (R = -0.32: P = 0.05). Omental ZFP36 mRNA abundance was also positively correlated with adiponectinemia (R = 0.35; P=0.03) but not with circulating TNF-alpha, IL-6, and resistin concentrations.Conclusion: These results suggest that ZFP36 gene expression in omental adipose tissue, but not in abdominal s.c. fat, may offer partial protection against the development of insulin resistance and diabetes.