Functional characterization of human IgG, IgM, and IgA antibody directed to the capsule of Haemophilus influenzae type b.
Functional characterization of human IgG, IgM, and IgA antibody directed to the capsule of Haemophilus influenzae type b.
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针对 b 型流感嗜血杆菌荚膜的人 IgG、IgM 和 IgA 抗体的功能特征。
DOI：
10.1093/infdis/153.1.8
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Siber,GR
中科院分区：
文献类型：
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作者：
Schreiber,JR;Barrus,V;Cates,KL;Siber,GR
抗流感嗜血杆菌（Hib）荚膜多糖（CP）抗体具有杀菌、调理和保护作用。根据被动免疫研究计算，Hib CP主要IgG抗体的最低保护水平为0.06 - 0.15 µg/ml血清。然而，人类对Hib胶囊抗原攻击的反应包括产生针对不同同种型Hib CP的抗体，其针对Hib的功能尚不清楚。为了表征不同同种型的Hib CP抗体的功能，我们从先前用纯化的Hib CP疫苗免疫的成年供体的血清中纯化人IgG、IgM和伊加。球蛋白制剂的同种型纯度>99%，含有大量的抗囊抗体，并且对Hib的功能不同。IgG抗体的Hib CP是杀菌和调理剂的人多形核白细胞（PMNLs）的存在下，补体和保护的婴儿大鼠。IgM，虽然比IgG更杀菌（P<0.01），在大鼠中同样具有保护作用，但对PMNL的Hib调理作用较差。伊加不是杀菌或调理剂，并没有防止菌血症和脑膜炎的大鼠挑战Hib。我们得出结论，针对Hib的胶囊的抗体根据类别的抗菌功能不同。这些数据可能是重要的，以准确地估计最低保护水平的抗囊抗体接种疫苗或自然感染后，并可能有影响的方式，其中主机清除Hib的循环。
Antibody to the capsular polysaccharide (CP) ofHaemophilus influenzaeb (Hib) is bactericidal, opsonic, and protective. Minimum protective levels of primarily IgG antibody to Hib CP, calculated from passive immunization studies, have been found to be ∼.06–.15 µg/ml of serum. The human response to antigenic challenge with the Hib capsule, however, includes production of antibody to Hib CP of different isotypes whose function against Hib is unclear. In order to characterize the function of antibody to Hib CP of different isotypes, we purified human IgG, IgM, and IgA from the serum of an adult donor who had been previously immunized with purified Hib CP vaccine. The globulin preparations were >99% isotypically pure, contained large quantities of anticapsular antibody, and differed in function against Hib. IgG antibody to Hib CP was bactericidal and opsonic for human polymorphonuclear leukocytes (PMNLs) in the presence of complement and protective in infant rats. IgM, although more bactericidal than IgG (P< .01) and equally protective in rats, opsonized Hib poorly for PMNLs. IgA was not bactericidal or opsonic and did not prevent bacteremia and meningitis in rats challenged with Hib. We conclude that antibody directed against the capsule of Hib differs in antibacterial function depending on class. These data may be important to acurately estimate minimum protective levels of anticapsular antibody after vaccination or natural infection and may have implications for the manner in which the host clears Hib from the circulation.