Temperature controls oxidative phosphorylation and reactive oxygen species production through uncoupling in rat skeletal muscle mitochondria
Temperature controls oxidative phosphorylation and reactive oxygen species production through uncoupling in rat skeletal muscle mitochondria
复制标题
DOI：
10.1016/j.freeradbiomed.2015.02.012
复制
发表时间：
2015-06-01
影响因子：
7.4
通讯作者：
Zoladz, Jerzy A.
中科院分区：
文献类型：
--
作者：
Jarmuszkiewicz, Wieslawa;Woyda-Ploszczyca, Andrzej;Zoladz, Jerzy A.
在实验诱导的低温（25℃）和高温（42℃）下，与静息肌肉的生理温度（35℃）相比，研究了离体大鼠骨骼肌线粒体的线粒体呼吸和磷酸化活动、线粒体解偶联和过氧化氢形成。对于非磷酸化线粒体，将温度从25摄氏度提高到42摄氏度会导致膜电位、过氧化氢产量和醌还原水平的降低。对于磷酸化线粒体，没有观察到这些线粒体功能的温度依赖性变化。然而，随着实验温度的升高，氧化磷酸化的效率降低，而线粒体的氧化和磷酸化速率和氧化能力增加。随着实验温度的升高，质子泄漏（包括解偶联蛋白介导的质子泄漏）增加，这可以解释氧化磷酸化效率和活性氧产生的降低。(C) 2015爱思唯尔公司版权所有。
Mitochondrial respiratory and phosphorylation activities, mitochondrial uncoupling, and hydrogen peroxide formation were studied in isolated rat skeletal muscle mitochondria during experimentally induced hypothermia (25 degrees C) and hyperthermia (42 degrees C) compared to the physiological temperature of resting muscle (35 degrees C). For nonphosphorylating mitochondria, increasing the temperature from 25 to 42 degrees C led to a decrease in membrane potential, hydrogen peroxide production, and quinone reduction levels. For phosphorylating mitochondria, no temperature-dependent changes in these mitochondrial functions were observed. However, the efficiency of oxidative phosphorylation decreased, whereas the oxidation and phosphorylation rates and oxidative capacities of the mitochondria increased, with increasing assay temperature. An increase in proton leak, including uncoupling protein-mediated proton leak, was observed with increasing assay temperature, which could explain the reduced oxidative phosphorylation efficiency and reactive oxygen species production. (C) 2015 Elsevier Inc. All rights reserved.