Long-term assessment of motor and cognitive behaviours in the intraluminal perforation model of subarachnoid hemorrhage in rats
Long-term assessment of motor and cognitive behaviours in the intraluminal perforation model of subarachnoid hemorrhage in rats
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DOI：
10.1016/j.bbr.2008.11.019
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发表时间：
2009-03-17
影响因子：
2.7
通讯作者：
Colbourne, Frederick
中科院分区：
文献类型：
--
作者：
Silasi, Gergely;Colbourne, Frederick
蛛网膜下腔出血（SAH）的血管内穿孔模型是大鼠中常用的模型，因为它在没有开颅术的情况下进行，并且准确地模拟了人类SAH的生理效应。然而，该模型的长期行为特征尚未得到描述。考虑到人类在SAH后经常有认知缺陷，我们着手描述大鼠在腔内穿孔后的行为特征以及自发温度变化。在接受SAH之前，对大鼠进行三项运动任务（锥形梁、肢体使用不对称和水平阶梯任务）的预训练。然后在手术后第3、7、14和21天评估动物的这些任务。在完成运动测试时，在移动平台版本的Morris水任务上评估大鼠。尽管有显著的死亡率（33%），但SAH并没有导致任何检查任务的持久运动缺陷。然而，SAH组在Morris水任务中确实表现出轻微的认知障碍。此外，SAH导致体温轻微但显著升高（与假手术大鼠相比），尽管一般饲养笼活动急剧减少。大多数动物没有任何可观察到的梗死，SAH没有显著影响皮质厚度。总之，SAH的血管内穿孔模型不会导致持久的运动缺陷，仅在幸存者中导致轻微的认知障碍，单独在神经保护或康复研究中难以评价。(C)2008 Elsevier B.V.保留所有权利。
The endovascular perforation model of subarachnoid hemorrhage (SAH) is a commonly used model in rats as it is performed without a craniotomy and accurately mimics the physiological effects of SAH in humans. The long-term behavioural profile of the model, however, has not been characterized. Given that humans often have cognitive deficits following SAH, we set out to characterize the behavioural profile as well as the spontaneous temperature changes of rats following intraluminal perforation. Rats were pre-trained on three motor tasks (tapered beam, limb-use asymmetry and the horizontal ladder tasks) prior to receiving a SAH. The animals were then assessed on post-surgical days 3, 7,14 and 21 on these tasks. At the completion of motor testing, the rats were assessed on a moving platform version of the Morris water task. Despite significant mortality (33%), SAH did not result in lasting motor deficits on any of the tasks examined. However, the SAH group did show a minor cognitive impairment in the Morris water task. In addition, SAH produced a slight, but significant elevation in body temperature (vs. sham operated rats) despite an acute decrease in general home cage activity. The majority of the animals did not have any observable infarcts and the SAH did not significantly affect cortical thickness. In summary, the endovascular perforation model of SAH results in no lasting motor deficits and only minor cognitive impairment in survivors, which alone would be difficult to evaluate in neuroprotection or rehabilitation studies. (C) 2008 Elsevier B.V. All rights reserved.