SUBCELLULAR-LOCALIZATION OF THE 25-KDA PROTEIN ENCODED IN THE TRIPLE GENE BLOCK OF POTATO VIRUS-X
SUBCELLULAR-LOCALIZATION OF THE 25-KDA PROTEIN ENCODED IN THE TRIPLE GENE BLOCK OF POTATO VIRUS-X
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DOI：
10.1006/viro.1993.1577
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发表时间：
1993-11-01
期刊：
影响因子：
3.7
通讯作者：
BAULCOMBE, DC
中科院分区：
文献类型：
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作者：
DAVIES, C;HILLS, G;BAULCOMBE, DC
马铃薯X病毒（PVX）的25-kDa蛋白由重叠三重基因块的5 '开放阅读框编码，具有核苷酸结合蛋白特征的GKS序列基序，被认为在细胞之间的运动中发挥作用。我们已经产生了一个多克隆抗体的25 kDa的蛋白质，以研究其合成和细胞内的位置。在感染组织提取物的Western印迹上，该抗体检测到具有预期迁移率的单一主要蛋白质，该蛋白质比感染原生质体中的病毒外壳蛋白更早产生，并且主要位于感染细胞的1000 g沉淀级分中。感染组织的薄切片的免疫金标记显示，25 kDa的蛋白质与PVX感染的细胞质包涵体特征，但不与细胞壁或胞间连丝。因此，尽管突变研究表明25-kDa蛋白在病毒感染的细胞间运动中的作用，但它不太可能以与烟草花叶病毒和豇豆花叶病毒的其他充分表征的运动蛋白完全相同的方式起作用。
The 25-kDa protein of potato virus X (PVX) is encoded by the 5′ open reading frame of the overlapping triple gene block, has a GKS sequence motif characteristic of nucleotide binding proteins, and is thought to play a role in movement between cells. We have produced a polyclonal antibody to the 25-kDa protein to investigate its synthesis and intracellular location. On Western blots of infected tissue extracts this antibody detected a single major protein of the expected mobility, produced earlier than the viral coat protein in infected protoplasts and located predominantly in the 1000 g pellet fraction of infected cells. Immunogold labeling of thin sections of infected tissue revealed that the 25-kDa protein is associated with the cytoplasmic inclusion bodies characteristic of PVX infections, but not with the cell wall or plasmodesmata. Therefore, although mutational studies indicate a role for the 25-kDa protein in the cell to cell movement of viral infection it is unlikely to act in precisely the same manner as the other well-characterized movement proteins of tobacco mosaic and cowpea mosaic viruses.