Human ACE gene polymorphism and distilled water induced cough
Human ACE gene polymorphism and distilled water induced cough
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DOI：
10.1136/thx.52.2.111
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发表时间：
1997-02-01
期刊：
影响因子：
10
通讯作者：
Linton, K
中科院分区：
文献类型：
--
作者：
Morice, AH;Turley, AJ;Linton, K
背景-血管紧张素转换酶(ACE)的抑制剂会导致无效的咳嗽。以血管紧张素转换酶基因插入/缺失多态性为遗传标记，探讨血管紧张素转换酶基因与咳嗽敏感性的关系。方法采用蒸馏水雾化吸入对66例正常血压受试者(男34例)进行双盲咳嗽激发，年龄18~80岁，平均34.8岁。记录暴露在水中一分钟内的咳嗽次数。通过聚合酶链式反应从静脉血中扩增DNA样本，并在1%的琼脂糖凝胶上进行分离。分析血管紧张素转换酶基因内含子16的插入(I)或缺失(D)的多态现象，其中插入或缺失一个Alu重复序列287个碱基对。咳嗽反应明显优于对照组(P<0.05)。
Background - Inhibitors of angiotensin converting enzyme (ACE) cause a non-productive cough. The insertion/deletion polymorphism of ACE was used as a genetic marker to investigate the relationship between ACE genotype and cough sensitivity.Methods - A double blind cough challenge was performed in 66 normotensive subjects (34 men) of mean age 34.8 years (range 18-80) using aerosols of distilled water. The number of coughs during the one minute exposure to water was recorded. DNA samples from venous blood were amplified by the polymerase chain reaction and resolved on a 1% agarose gel. They were analysed for the presence of a polymorphism in intron 16 of the ACE gene consisting of an insertion (I) or deletion (D) of an Alu repetitive sequence 287 base pairs long.Results - The distribution of genotypes was 20 II, 26 ID, and 20 DD. The cough response was significantly (p