Localization of the calcium channel subunits Cav1.2 (alpha1C) and Cav2.3 (alpha1E) in the mouse organ of Corti.
Localization of the calcium channel subunits Cav1.2 (alpha1C) and Cav2.3 (alpha1E) in the mouse organ of Corti.
复制标题
小鼠柯蒂氏器官中钙通道亚基 Cav1.2 (alpha1C) 和 Cav2.3 (alpha1E) 的定位。
DOI：
10.14670/hh-18.1115
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发表时间：
2003
影响因子：
2
通讯作者：
J. Engel
中科院分区：
文献类型：
--
作者：
N. Waka;M. Knipper;J. Engel
电压激活的Ca ~（2+）通道在突触传递、信号处理和发育中起着重要作用。使用亚基特异性抗体和荧光二抗以及耳蜗冷冻切片，研究了发育和成年小鼠Corti器官中Cav1.2（alpha 1C）和Cav2.3（alpha 1 E）Ca 2+通道的免疫组织化学定位。Cav1.2免疫反应性已被检测到从出生后第14天（P14）起，在胆碱能内侧传出神经和外毛细胞之间的突触所揭示的抗突触素和抗胆碱乙酰转移酶的共染色。最有可能的Cav1.2免疫反应性位于突触前的传出终扣与外毛细胞，这表明C类L-型钙离子通道在内侧传出系统的突触传递的作用的接触的网站。第二个钙离子通道Cav2.3的定位在耳蜗发育过程中表现出明显的变化。从P2到P10，Cav2.3免疫反应被发现在外螺旋束，其次是内螺旋束，传出末梢和内侧传出纤维。在P14前后，Cav2.3免疫反应性从这些结构中消失，并且从P19开始，在外毛细胞膜的基底极中观察到Cav2.3免疫反应性。
Voltage-activated Ca2+ channels play an important role in synaptic transmission, signal processing and development. The immunohistochemical localization of Cav1.2 (alpha1C) and Cav2.3 (alpha1E) Ca2+ channels was studied in the developing and adult mouse organ of Corti using subunit-specific antibodies and fluorescent secondary antibodies with cochlear cryosections. Cav1.2 immunoreactivity has been detected from postnatal day 14 (P14) onwards at the synapses between cholinergic medial efferents and outer hair cells as revealed by co-staining with anti-synaptophysin and anti-choline acetyltransferase. Most likely the Cav1.2 immunoreactivity was located presynaptically at the site of contact of the efferent bouton with the outer hair cell which suggests a role for class C L-type Ca2+ channels in synaptic transmission of the medial efferent system. The localization of the second Ca2+ channel tested, Cav2.3, showed a pronounced change during cochlear development. From P2 until P10, Cav2.3 immunoreactivity was found in the outer spiral bundle followed by the inner spiral bundle, efferent endings and by medial efferent fibers. Around P14, Cav2.3 immunoreactivity disappeared from these structures and from P19 onwards it was observed in the basal poles of the outer hair cell membranes.