Pectin acetylesterase PAE5 is associated with the virulence of plant pathogenic oomycete Peronophythora litchii
Pectin acetylesterase PAE5 is associated with the virulence of plant pathogenic oomycete Peronophythora litchii
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果胶乙酰酯酶 PAE5 与植物病原卵菌荔枝霜霉的毒力相关
DOI：
10.1016/j.pmpp.2018.11.006
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发表时间：
2019-04-01
影响因子：
2.7
通讯作者：
Jiang, Zide
中科院分区：
文献类型：
--
作者：
Kong, Guanghui;Wan, Lang;Jiang, Zide
果胶是高等植物初生细胞壁的主要成分。果胶中的一些半乳糖醛基残基常被o -乙酰化。参与果胶酶解乙酰化的果胶乙酰酯酶（PAEs）广泛存在于植物致病卵菌中，并表现出序列和转录多态性。荔枝疫霉（Peronophythora litchii）是已知对荔枝（Litchi chinensis Sonn）最具破坏性的病原体。从荔枝、疫霉和霉属的38个PAE基因序列分析中，筛选出5个PAE基因进行进一步研究。其中，利用CRISPR/Cas9技术敲除荔枝PAE4和PAE5，命名为PlPAE4和PlPAE5。用荔枝植株接种试验证实，plpae5突变体的侵染能力低于野生型荔枝菌株SHS3。PlPAE5在本菌烟中的异源表达可促进辣椒疫霉菌的侵染。这些结果表明PlPAE5在卵菌致病性中起重要作用。
Pectin is a major component of the primary cell wall of higher plants. Some galacturonyl residues in pectin are often O-acetylated. Pectin acetylesterases (PAEs) involved in enzymatic deacetylation of pectin are widespread in the plant pathogenic oomycetes and show sequence and transcriptional polymorphism. Peronophythora litchii is the most destructive pathogens known on lychee (Litchi chinensis Sonn). Out of the 38 PAEs predicted from sequence analysis of P. litchii, Phytophthora species and Pythium species, five P. litchii PAE genes were selected for further study. Of these, P. litchii PAE4 and PAE5, named PlPAE4 and PlPAE5, were knocked out using CRISPR/Cas9 technique. The plpae5 mutants were less capable of host invasion than the wild-type P. litchii strain SHS3, as tested by lychee plant inoculation. Heterologous expression of PlPAE5 in Nicotiana benthamiana could promote the infection by Phytophthora capsici. These results demonstrate that PlPAE5 plays an important role in oomycete pathogenicity.