Granulocyte-macrophage colony-stimulating factor (GM-CSF): a chemoattractive agent for murine leukocytes in vivo
Granulocyte-macrophage colony-stimulating factor (GM-CSF): a chemoattractive agent for murine leukocytes in vivo
复制标题
DOI：
10.1189/jlb.0809546
复制
发表时间：
2011-06-01
影响因子：
5.5
通讯作者：
McCafferty, Donna-Marie
中科院分区：
文献类型：
--
作者：
Khajah, Maitham;Millen, Brandie;McCafferty, Donna-Marie
GM-CSF被认为是造血细胞的增殖剂，并对中性粒细胞起启动作用。本研究的目的是确定GM-CSF在体内是否具有中性粒细胞趋化剂的作用，以及它是否有助于肠道炎症期间的募集。体外初步研究，使用琼脂糖凝胶测定，确定GM-CSF可以诱导中性粒细胞迁移，其摩尔浓度远低于fmlp样肽WKYMVm （33.5-134 nM vs 1-10 μ M）。gm - csf诱导的中性粒细胞迁移被消融(
GM-CSF is well recognized as a proliferative agent for hematopoietic cells and exerts a priming function on neutrophils. The aim of this study was to determine if GM-CSF has a role as a neutrophil chemoattractant in vivo and if it can contribute to recruitment during intestinal inflammation. Initial studies in vitro, using the under-agarose gel assay, determined that GM-CSF can induce neutrophil migration at a much lower molar concentration than the fMLP-like peptide WKYMVm (33.5-134 nM vs. 1-10 mu M). GM-CSF-induced neutrophil migration was ablated (