DIFFERENTIAL EXPRESSION OF FACTOR-XIIIA AND CD34 IN CUTANEOUS MESENCHYMAL TUMORS
DIFFERENTIAL EXPRESSION OF FACTOR-XIIIA AND CD34 IN CUTANEOUS MESENCHYMAL TUMORS
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DOI：
10.1111/j.1600-0560.1993.tb00233.x
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发表时间：
1993-04-01
影响因子：
1.7
通讯作者：
FIVENSON, DP
中科院分区：
文献类型：
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作者：
ALTMAN, DA;NICKOLOFF, BJ;FIVENSON, DP
真皮各种树突状细胞之间的组织发生关系尚不清楚，这些树突状细胞可能表达包括XIIIA(FXIIIa)或CD34在内的标记。在这项研究中，我们利用灵敏的间接免疫过氧化物酶染色技术检测CD34和FXIIIa以及单核/巨噬细胞标记物KP-1和MAC387在各种间叶性皮肤肿瘤中的表达，以了解CD34和FXIIIa是否存在差异表达。肿瘤包括皮肤纤维瘤(DF)10例，瘢痕疙瘩(N=9)，不典型纤维黄瘤(AFX)3例，隆起皮肤纤维肉瘤(DFSP)7例。DF均由FXIIIa+梭形和星状肿瘤细胞组成(平均评分=4.9或大于等于75%)，但这些细胞很少表达CD34(<；10%)。7例DFSP中6例CD34+和FXIIa均为阴性，1例CD34和FXIIa均为阴性。在所有DFSP中，均有截留的FXIIIa+细胞，与梭形肿瘤细胞不同。AFX显示间质中FXIIIa+细胞数量稀少(平均为1.33分或10-25%阳性)，这与这些肿瘤的不典型巨细胞不同。瘢痕疙瘩中同样含有截留的FXIIIa+细胞(平均分数为0.44或5%阳性)，与肿块中的纺锤形成纤维细胞不同。这些肿瘤中的树突状细胞和梭形细胞始终为KP-1和Mac-387阴性，而所有研究的肿瘤都以散在圆形的组织细胞为特征，无论肿瘤细胞类型如何，这些细胞都是KP-1+和/或Mac-387+。我们认为，这些肿瘤可以通过FXIIIa和CD34表达的相对程度来区分，这些肿瘤可能是研究CD34+细胞和真皮树突状细胞关系的有用纽带。
The histogenetic relationship amongst various dendritic cells of the dermis which may express markers including factor XIIIa (FXIIIa) or CD34 remains unclear. In this study we utilized a sensitive indirect immunoperoxidase staining technique to identify CD34 and FXIIIa, as well the monocyte/macrophage markers KP-1 and MAC 387 expression in a variety of cutaneous dermal tumors of mesenchymal origin to see if differential expression of CD34 vs FXIIIa exists. Tumors studied included dermatofibroma (DF) (N = 10), keloid (N = 9), atypical fibroxanthoma (AFX) (N = 3), and dermatofibrosarcoma protuberans (DFSP) (N = 7). DF were all composed of FXIIIa+ spindle-shaped and stellate tumor cells (mean score = 4.9 or greater-than-or-equal-to 75% FXIIIa+) as previously reported, but these cells rarely (< 10%) expressed CD34. Six of 7 DFSP were found to be > 75% CD34+ and FXIIIa negative, while one DFSP was negative for both CD34 and FXIIIa. In all DFSP, there were trapped FXIIIa+ cells which were distinct from the spindle-shaped tumor cells. AFX showed sparse populations of FXIIIa+ cells in the stroma (mean score = 1.33 or 10-25% positive), which were distinct from the atypical giant cells characteristic of these tumors. Keloid similarly contained trapped FXIIIa+ cells (mean score = 0.44 or < 5% positive) that were distinct from the spindle-shaped fibroblasts of the tumor mass. Dendritic and spindle-shaped cells within these tumors were consistently both KP-1 and Mac-387 negative, while all lesions studied were characterized by scattered round, histiocytic cells which were KP-1+ and/or Mac-387+ irrespective of tumor cell type. We suggest that these tumors can be delineated by their relative degrees of FXIIIa and CD34 expression and that these neoplasms may be a useful link with which to study the relationship between CD34+ cells and dermal dendrocytes.