Magnetically enriched bone marrow-derived macrophages loaded in vitro with iron oxide can migrate to inflammation sites in mice
Magnetically enriched bone marrow-derived macrophages loaded in vitro with iron oxide can migrate to inflammation sites in mice
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DOI：
10.1002/nbm.1168
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发表时间：
2008-02-01
影响因子：
2.9
通讯作者：
Silengo, Lorenzo
中科院分区：
文献类型：
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作者：
Hamm, Joerg;Pulito, Roberta;Silengo, Lorenzo
细胞的体外标记允许大量的氧化铁掺入，因此具有很高的检测灵敏度，但标记的细胞是否会对刺激做出正常反应仍存在争议。这个问题是通过在体外分化骨髓来源的巨噬细胞(BMDM)，在体外用高浓度的Endorem标记细胞，以及通过磁浓缩消除未标记的细胞来解决的。为了探索它们的急性炎症反应，将浓缩的细胞注射到角叉菜胶致炎症的小鼠体内，这是一种“气囊模型”。尾静脉注射BMDM 16h后，对炎症部位恢复的细胞进行体外MRI和荧光显微镜观察。两种检测方法均可检测到Endorem标记的细胞。这表明，体外负载高浓度氧化铁的BMDM仍然可以对趋化因子梯度做出反应，并渗透到小鼠的炎症组织中。此外，通过使用转基因小鼠作为BMDM捐赠者，应该可以研究单个基因在巨噬细胞募集中的作用。版权所有(C)2007 John Wiley&Sons，Ltd.
In vitro labelling of cells permits incorporation of large amounts of iron oxide and consequently high detection sensitivity, but it remains controversial whether labelled cells would respond normally to stimuli. This question was addressed by differentiating bone marrow-derived macrophages (BMDMs) in vitro, labelling cells with high concentrations of Endorem in vitro, and eliminating unlabelled cells by magnetic enrichment. To explore their acute inflammatory response, enriched cells were injected into mice with carrageenan-induced inflammation, the 'air pouch model'. Cells recovered from the inflammation site 16 h after intravenous BMDM injection into the tail vein were analysed by in vitro MRI and fluorescent microscopy. With both assays, Endorem-labelled cells were detectable. This indicates that BMDMs, loaded with high concentrations of iron oxide in vitro, can still respond to chemokine gradients and infiltrate inflamed tissue in mice. Furthermore, by using genetically modified mice as BMDM donors, it should be possible to study the role of individual genes in macrophage recruitment. Copyright (C) 2007 John Wiley & Sons, Ltd.