Evaluation of PCR for detection of Campylobacter in a national broiler surveillance programme in Denmark
Evaluation of PCR for detection of Campylobacter in a national broiler surveillance programme in Denmark
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DOI：
10.1046/j.1365-2672.2003.01934.x
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发表时间：
2003-01-01
影响因子：
4
通讯作者：
Madsen, M
中科院分区：
文献类型：
--
作者：
Lund, M;Wedderkopp, A;Madsen, M
目的：建立一种快速、灵敏的弯曲杆菌PCR检测方法。方法和结果：使用磁珠从粪便拭子中分离DNA，然后使用预先等分的PCR混合物进行PCR，该混合物已储存在冰箱中。结果可以在
Aims: To develop and evaluate a rapid and sensitive PCR method for detection of Campylobacter spp. directly from chicken faeces.Methods and Results: DNA was isolated from faecal swabs using magnetic beads followed by PCR using a prealiquoted PCR mixture, which had been stored in the freezer. The result could be obtained in