ISOLATION OF BIOLOGICALLY-ACTIVE RIBONUCLEIC-ACID FROM SOURCES ENRICHED IN RIBONUCLEASE
ISOLATION OF BIOLOGICALLY-ACTIVE RIBONUCLEIC-ACID FROM SOURCES ENRICHED IN RIBONUCLEASE
复制标题
DOI：
10.1021/bi00591a005
复制
发表时间：
1979-01-01
期刊：
影响因子：
2.9
通讯作者：
RUTTER, WJ
中科院分区：
文献类型：
--
作者：
CHIRGWIN, JM;PRZYBYLA, AE;RUTTER, WJ
通过在有效蛋白质变性硫氰酸硫氰酸硫氰酸盐的4 m溶液中加上0.1 m 2-巯基乙醇中的4 m溶液中有效匀浆，从富含RNase（例如大鼠胰腺的组织）制备完整的RNA。通过乙醇沉淀或通过CSCL沉降将RNA无蛋白质分离。通过这些手段获得的大鼠胰腺RNA用作纯化.alpha.-淀粉酶mRNA的来源。
Intact RNA was prepared from tissues rich in RNase such as the rat pancreas by efficient homogenization in a 4 M solution of the potent protein denaturant guanidinium thiocyanate plus 0.1 M 2-mercaptoethanol to break protein disulfide bonds. The RNA was isolated free of protein by ethanol precipitation or by sedimentation through CsCl. Rat pancreas RNA obtained by these means was used as a source for the purification of .alpha.-amylase mRNA.