Antimicrobial Activity of Lactobacillus Species Against Carbapenem-Resistant Enterobacteriaceae
Antimicrobial Activity of Lactobacillus Species Against Carbapenem-Resistant Enterobacteriaceae
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DOI：
10.3389/fmicb.2019.00789
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发表时间：
2019-04-18
影响因子：
5.2
通讯作者：
Tang, Hung-Jen
中科院分区：
文献类型：
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作者：
Chen, Chi-Chung;Lai, Chih-Cheng;Tang, Hung-Jen
目的：本研究的目的是确定合适的乳酸杆菌，具有抗碳青霉烯耐药肠杆菌科（CRE）的活动，在体外胃蛋白酶和胆盐的耐受性。收集了包括9个种的菌株用于调查。它们在胃蛋白酶和胆盐存在下的活力使用耐受性试验进行测试。他们的抗CRE效果进行了评估，通过琼脂扩散和肉汤微量稀释法，以及时间杀死test.Results：收集的57株乳杆菌中，31个有不到2个对数减少，在胃蛋白酶和胆盐耐受性试验中，他们的生存能力。在这31株分离株中，5株（LUC 0180、LUC 0219、LYC 0289、LYC 0413和LYC 1031）显示出最大的抗CRE活性，在琼脂孔扩散试验中CRE抑制区大于15 mm。这五种菌株的上清液对克雷斯的最小抑制百分比范围为10 - 30%。除LUC 0180具有>= 40%的最小杀菌百分比外，所有菌株的杀菌百分比范围为20 - 40%。来自这些乳杆菌属菌株的无细胞培养上清液的抑制作用在加热后没有改变，但随着pH变为7.0而消失。孵育24小时后，浓度为10（8）CFU/ml的5种乳杆菌菌株完全抑制碳青霉烯类耐药大肠杆菌（CRE 316）和肺炎克雷伯氏菌（CRE 632）的生长。培养48 h后，基于时间杀灭试验，在各浓度的乳酸杆菌下，CRE 316的生长被完全抑制。此外，当乳杆菌浓度为10（8）CFU/ml时，pH下降速度比其他浓度更快。结论：部分乳杆菌菌株具有抗CRE活性，这表明其在控制或预防CRE定植或感染方面具有潜在的应用。
Objective: This study aims to identify suitable lactobacilli that have anti-carbapenemresistant Enterobacteriaceae (CRE) activity with in vitro tolerance to pepsin and bile salts.Methods: Fifty-seven Lactobacillus spp. strains encompassing nine species were collected for investigation. Their viabilities in the presence of pepsin and bile salts were tested using tolerance tests. Their anti-CRE effects were assessed by agar well diffusion and broth microdilution assay, as well as time-kill test.Results: Of the 57 Lactobacillus isolates collected, 31 had a less than 2-log reduction in their viability in both pepsin and bile salt tolerance tests. Of these 31 isolates, 5 (LUC0180, LUC0219, LYC0289, LYC0413, and LYC1031) displayed the greatest anti-CRE activity with a CRE zone of inhibition greater than 15 mm in agar well diffusion assays. The minimal inhibitory percentages of supernatants from these five strains against CREs ranged from 10 to 30%. With the exception of LUC0180, which had a minimal bactericidal percentage >= 40%, the bactericidal percentage of all the strains ranged from 20 to 40%. The inhibitory effect of the cell-free culture supernatants from these Lactobacillus strains did not change after heating but was abolished as the pH changed to 7.0. After a 24-h incubation, five of the Lactobacillus strains at a concentration of 10(8) CFU/ml totally inhibited the growth of carbapenem-resistant Escherichia coil (CRE316) and Kiebsiella pneumoniae (CRE632). After a 48-h incubation, the growth of CRE316 was completely inhibited under each concentration of lactobacilli based on time-kill test. Furthermore, when the concentration of lactobacilli was at 10(8) CFU/ml, the decline in pH was faster than at other concentrations.Conclusion: Some Lactobacillus strains exhibit anti-CRE activity, which suggests potential applications for controlling or preventing CRE colonization or infection.