Intrauterine occupancy controls expression of the parathyroid hormone-related peptide gene in preterm rat myometrium.
Intrauterine occupancy controls expression of the parathyroid hormone-related peptide gene in preterm rat myometrium.
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DOI：
10.1073/pnas.87.18.6969
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发表时间：
1990-09
影响因子：
11.1
通讯作者：
M. A. Thiede;A G Daifotis;E. C. Weir;M L Brines;W. Burtis;K. Ikeda;B. E. Dreyer;R E Garfield;A. Broadus
中科院分区：
文献类型：
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作者：
M. A. Thiede;A G Daifotis;E. C. Weir;M L Brines;W. Burtis;K. Ikeda;B. E. Dreyer;R E Garfield;A. Broadus
甲状旁腺激素（PTH）和甲状旁腺相关肽（PTHRP）基因是基因家族的成员。而PTH是一个经典的肽类激素，越来越多的证据表明，PTHRP可能有主导的局部行动。我们在这里报告，PTHRP基因表达在大鼠子宫肌层，与PTHRP mRNA表达的主峰发生在48小时立即分娩前。生物学和免疫学检测发现，在组织提取物中的肽含量的一个类似的峰值，但PTHRP不能检测到外周循环或子宫静脉血浆在妊娠晚期。通过原位杂交组织化学，PTHRP mRNA被证明在纵向和环形层的平滑肌，但在子宫内膜缺席。子宫肌层PTHRP mRNA在妊娠晚期的上升依赖于宫内占位，它大大减少或不存在于非妊娠子宫角。这些结果表明，PTHRP基因在早产子宫肌层的表达是在一个局部刺激的控制下，并建议，PTHRP可能发挥旁分泌或自分泌的作用，在子宫在产前期间，可能涉及子宫肌层收缩。
The parathyroid hormone (PTH) and parathyroid hormone-related peptide (PTHRP) genes are members of a gene family. Whereas PTH is a classical peptide hormone, mounting evidence suggests that the PTHRP may have predominately local actions. We report here that the PTHRP gene is expressed in rat myometrium, with a major peak in PTHRP mRNA expression occurring in the 48 hr immediately preceding parturition. A similar peak in peptide content was found in tissue extracts by biological and immunological assays, but the PTHRP could not be detected in the peripheral circulation or in uterine vein plasma during late gestation. By in situ hybridization histochemistry, PTHRP mRNA was demonstrated in both the longitudinal and circular layers of smooth muscle but was absent in the endometrium. The rise in myometrial PTHRP mRNA in late gestation was dependent upon intrauterine occupancy; it was greatly reduced or absent in nongravid uterine horns. These findings indicate that the expression of the PTHRP gene in preterm myometrium is under the control of a local stimulus and suggest that the PTHRP may play a paracrine or autocrine role in the uterus during the antepartum period, possibly involving myometrial contractility.