Relative rates of non-pneumonic SARS coronavirus infection and SARS coronavirus pneumonia.
Relative rates of non-pneumonic SARS coronavirus infection and SARS coronavirus pneumonia.
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DOI：
10.1016/s0140-6736(04)15729-2
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发表时间：
2004-03-13
期刊：
影响因子：
--
通讯作者：
Yuen KY
中科院分区：
文献类型：
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作者：
Woo PC;Lau SK;Tsoi HW;Chan KH;Wong BH;Che XY;Tam VK;Tam SC;Cheng VC;Hung IF;Wong SS;Zheng BJ;Guan Y;Yuen KY
虽然严重急性呼吸道综合征冠状病毒（SARS-CoV）的基因组已经测序，并确定了一个可能的动物水库，血清阳性率研究和大规模筛查检测亚临床和非肺炎感染仍然缺乏。本研究克隆并纯化了SARS冠状病毒核蛋白和刺突蛋白，用SARS冠状病毒肺炎患者血清检测了它们的免疫原性。用149例献血3年以上的健康献血员血清和106例SARS冠状病毒肺炎患者血清（间接免疫荧光法）检测了重组核衣壳蛋白IgG的ELISA检测方法。采用ELISA方法对香港SARS爆发期间献血的健康献血者、非肺炎住院患者和无肺炎的医护人员进行了SARS-CoV血清阳性率研究。所有阳性样品均通过两种单独的蛋白质印迹试验（重组核衣壳蛋白和重组刺突多肽）确认。Western-blot分析表明，SARS-CoV的核衣壳蛋白和刺突多肽具有很强的免疫原性。IgG抗体试验（ELISA法）的特异性为100%，敏感性为94.3%。在SARS爆发期间献血的400名健康献血者中有3名和131名非肺炎儿科住院患者中有1名IgG抗体阳性，经两种蛋白质印迹试验证实（总计占我们研究人群的0.48%）。我们的研究结果支持存在亚临床或非肺炎SARS-CoV感染。这种感染在我们当地比SARS冠状病毒肺炎更常见。
Although the genome of severe acute respiratory syndrome coronavirus (SARS-CoV) has been sequenced and a possible animal reservoir identified, seroprevalence studies and mass screening for detection of subclinical and non-pneumonic infections are still lacking. We cloned and purified the nucleocapsid protein and spike polypeptide of SARS-CoV and examined their immunogenicity with serum from patients with SARS-CoV pneumonia. An ELISA based on recombinant nucleocapsid protein for IgG detection was tested with serum from 149 healthy blood donors who donated 3 years previously and with serum positive for antibodies against SARS-CoV (by indirect immunofluorescence assay) from 106 patients with SARS-CoV pneumonia. The seroprevalence of SARS-CoV was studied with the ELISA in healthy blood donors who donated during the SARS outbreak in Hong Kong, non-pneumonic hospital inpatients, and symptom-free health-care workers. All positive samples were confirmed by two separate western-blot assays (with recombinant nucleocapsid protein and recombinant spike polypeptide). Western-blot analysis showed that the nucleocapsid protein and spike polypeptide of SARS-CoV are highly immunogenic. The specificity of the IgG antibody test (ELISA with positive samples confirmed by the two western-blot assays) was 100%, and the sensitivity was 94·3%. Three of 400 healthy blood donors who donated during the SARS outbreak and one of 131 non-pneumonic paediatric inpatients were positive for IgG antibodies, confirmed by the two western-blot assays (total, 0·48% of our study population). Our findings support the existence of subclinical or non-pneumonic SARS-CoV infections. Such infections are more common than SARS-CoV pneumonia in our locality.