The genes and mRNA coding for the heavy chains of chick embryonic skeletal myosin
The genes and mRNA coding for the heavy chains of chick embryonic skeletal myosin
复制标题
编码鸡胚骨骼肌球蛋白重链的基因和mRNA
DOI：
10.1016/0092-8674(77)90125-8
复制
发表时间：
1977
期刊：
影响因子：
64.5
通讯作者：
H. A. John
中科院分区：
文献类型：
--
作者：
M. Patrinou;H. A. John
A size class of’polysomes was isolated from chick embryonic leg skeletal muscle which synthesized almost exclusively a polypeptide chain with a molecular weight identical to the myosin heavy chain. The mRNA purified from these polysomes was shown to synthesize the 200,000 dalton polypeptide in the wheat germ cell-free translation system. At least 90% of the polypeptide had properties similar to the myosin heavy chain. Isoelectric focusing indicated that the myosin heavy chain synthesized in vitro contained two chains in equal amounts, as did purified embryonic leg skeletal muscle myosin. The kinetics of hybridization of the complementary DNA with an excess of the myosin heavy chain mRNA (MHC mRNA) indicated the presence of two different mRNA sequences. Reassociation of the cDNA to an excess of the DNA of the genome suggested that there is little, if any, reiteration of the myosin heavy chain genes.
