Alleviation of postharvest chilling injury in anthurium cut flowers by salicylic acid treatment
Alleviation of postharvest chilling injury in anthurium cut flowers by salicylic acid treatment
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DOI：
10.1016/j.scienta.2016.02.025
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发表时间：
2016-04-20
影响因子：
4.3
通讯作者：
Malekzadeh, Parviz
中科院分区：
文献类型：
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作者：
Aghdam, Morteza Soleimani;Jannatizadeh, Abbasali;Malekzadeh, Parviz
由于红掌花对冷害的敏感性，其最适贮藏温度为12.5-20 ℃。采后低温胁迫下活性氧（ROS）的积累导致膜完整性的丧失，这与多酚氧化酶（PPO）对酚类物质的氧化有关，而PPO是导致布朗宁的酶。苯丙氨酸解氨酶（PAL）是一种在低温胁迫下积累酚类物质的酶，较高的PAL酶活性可以作为植物抵御低温胁迫的一种防御机制。以红掌（Anthuriumandraeanumcv.）为试材，研究了不同浓度水杨酸（0、1、2和4 mM）处理对采后红掌冷害的影响。对切花在4 ℃下贮藏21 d的保鲜效果进行了研究。SA处理可延缓佛焰苞的布朗宁，抑制电解质渗漏和丙二醛（MDA）的增加。SA处理的红掌切花在4 ℃贮藏21 d时，PAL酶活性显著升高，PPO活性显著降低，总酚积累量和DPPH自由基清除率显著升高。SA处理的红掌切花具有较高的DPPH自由基清除活性，这可能与其抗氧化酶超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、抗坏血酸过氧化物酶（APX）和谷胱甘肽还原酶（GR）的活性有关。此外，SA处理增强了内源脯氨酸和甜菜碱（GB）的积累在红掌切花在4 ℃贮藏期间，这是与较高的佛焰苞相对含水量（RWC）。这些结果表明，SA处理可以作为一个有用的技术，通过提高总酚，脯氨酸，GB积累与提高抗氧化系统的活性，从而导致更高的膜完整性表现为较低的电解质渗漏和MDA含量的红掌切花采后冷害缓解。(C)© 2016 Elsevier B. V.版权所有。
Due to the sensitivity of anthurium flowers to chilling injury, its optimum temperature storage is 12.5-20 degrees C. Reactive oxygen species (ROS) accumulation during postharvest chilling stress leads to loss of membrane integrity which can be coincided with phenols oxidation by polyphenol oxidase (PPO) activity, the enzyme responsible for browning. Higher phenylalanine ammonia-lyase (PAL) activity, the enzyme responsible for phenols accumulation, under chilling stress, can act as a defense mechanism overcome chilling stress. In this experiment, the impact of 0, 1, 2 and 4 mM salicylic acid (SA) treatment applied by postharvest stem-end dipping (15 min at 20 degrees C) on chilling injury of Anthurium andraeanum cv. Sirion cut flowers storage at 4 degrees C for 21 days were investigated. SA treatment delayed spathe browning and retards electrolyte leakage and malondialdehyde (MDA) increase. The SA treated anthurium cut flowers exhibited significantly higher PAL enzyme activity, associated with lower PPO activity, which were coincided with higher total phenol accumulation and higher DPPH scavenging activity during storage at 4 degrees C for 21 days. Also, higher DPPH scavenging activity in anthurium cut flowers treated with SA can be results from higher antioxidant enzymes superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX) and glutathione reductase (GR) activity. Also, SA treatment enhanced endogenous proline and glycine betaine (GB) accumulation in anthurium cut flowers during storage at 4 degrees C, which was coincided with higher spathe relative water content (RWC). These results suggested that SA treatment can be used as a useful technology for the alleviation of postharvest chilling injury in anthurium cut flowers by enhancing total phenol, proline, and GB accumulation coincide with enhancing antioxidant system activity leading to higher membrane integrity showed by lower electrolyte leakage and MDA content. (C) 2016 Elsevier B.V. All rights reserved.