Genetic variation among species, races, forms and inbred lines of lac insects belonging to the genus Kerria (Homoptera, Tachardiidae).
Genetic variation among species, races, forms and inbred lines of lac insects belonging to the genus Kerria (Homoptera, Tachardiidae).
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DOI：
10.1590/s1415-47572011000300023
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发表时间：
2011-07
影响因子：
2.1
通讯作者：
Ramani R
中科院分区：
文献类型：
--
作者：
Ranjan SK;Mallick CB;Saha D;Vidyarthi AS;Ramani R
紫胶昆虫（同翅目：紫胶蝇科），属于紫胶属，被商业开发用于生产紫胶。紫堇是印度最常用的品种。利用RAPD标记对48个Kerria品系的遗传变异进行了分析，重点分析了lacca地理种族、亚亚特异形态、栽培品系、自交系等的遗传变异。在研究的48个品系中，26条RAPD引物共产生173个位点，多态性为97.7%。通过邻居连接，由相似矩阵生成的树状图将直线基本划分为两个簇和外群。主要类群由32个品系组成，主要包括兰属栽培品系、地理人种和自交系。第二组包括八种K. lacca，七种kusmi形式和一种来自南部卡纳塔克邦的rangeeni。剩下的8个系形成了一系列的外群，其中包括一组3个黄色突变系的K. lacca和其他被研究的Kerria物种。与来自同一种群的野生型相比，颜色突变体总是表现出独特的带状图案。本研究也为库斯米和rangeeni作为鹿角虱的亚种形式的现状提供了支持。
The lac insects (Homoptera: Tachardiidae), belonging to the genus Kerria, are commercially exploited for the production of lac. Kerria lacca is the most commonly used species in India. RAPD markers were used for assessing genetic variation in forty-eight lines of Kerria, especially among geographic races, infrasubspecific forms, cultivated lines, inbred lines, etc., of K. lacca. In the 48 lines studied, the 26 RAPD primers generated 173 loci, showing 97.7% polymorphism. By using neighbor-joining, the dendrogram generated from the similarity matrix resolved the lines into basically two clusters and outgroups. The major cluster, comprising 32 lines, included mainly cultivated lines of the rangeeni form, geographic races and inbred lines of K. lacca. The second cluster consisted of eight lines of K. lacca, seven of the kusmi form and one of the rangeeni from the southern state of Karnataka. The remaining eight lines formed a series of outgroups, this including a group of three yellow mutant lines of K. lacca and other species of the Kerria studied, among others. Color mutants always showed distinctive banding patterns compared to their wild-type counterparts from the same population. This study also adds support to the current status of kusmi and rangeeni, as infraspecific forms of K. lacca.