Doxycycline induces Fas Fas ligand-mediated apoptosis in Jurkat T lymphocytes
Doxycycline induces Fas Fas ligand-mediated apoptosis in Jurkat T lymphocytes
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DOI：
10.1006/bbrc.1999.0929
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发表时间：
1999-07-05
影响因子：
3.1
通讯作者：
Baxter, BT
中科院分区：
文献类型：
--
作者：
Liu, J;Kuszynski, CA;Baxter, BT
四环素类药物已被用于治疗慢性炎症性疾病，这些疾病与类风湿关节炎和牙周病中观察到的炎症细胞局部浸润和基质破坏有关。Fas/Fas配体（FasL）介导的细胞凋亡在维持T淋巴细胞稳态和调节免疫应答中起重要作用。本研究表明强力霉素可抑制Jurkat T淋巴细胞增殖并诱导细胞凋亡。植物血凝素（PHA）激活的Jurkat细胞更容易受到多西环素诱导的凋亡。此外，多西环素诱导的凋亡与Jurkat细胞中Fas/FasL表达增加有关。强力霉素处理Jurkat细胞凋亡增加与Fast表达增加一致。这些结果表明，强力霉素可能通过Fas/ fasl介导的细胞凋亡消除活化的T淋巴细胞，从而下调某些疾病的炎症过程。(C) 1999学术出版社。
Tetracyclines have been used in the treatment of chronic inflammatory diseases associated with local infiltration of inflammatory cells and matrix destruction as observed in rheumatoid arthritis and periodontal disease. Fas/Fas ligand (FasL)-mediated apoptosis plays an important role in maintaining T lymphocyte homeostasis and modulating immune response. The present study demonstrates that doxycycline inhibits Jurkat T lymphocyte proliferation and induces apoptosis. The phytohemagglutinin (PHA)-activated Jurkat cells are more susceptible to doxycycline-induced apoptosis. Furthermore, doxycycline-induced apoptosis is associated with increased Fas/FasL expression in Jurkat cells. The increase of apoptosis in Jurkat cells treated with doxycycline is consistent with the increase of Fast expression. These results suggest that doxycycline may downregulate the in-flammatory process in certain diseases by eliminating activated T lymphocytes through Fas/FasL-mediated apoptosis. (C) 1999 Academic Press.