Integrated immunoassay using tuneable surface acoustic waves and lensfree detection
Integrated immunoassay using tuneable surface acoustic waves and lensfree detection
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DOI：
10.1039/c1lc20320g
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发表时间：
2011-01-01
期刊：
影响因子：
6.1
通讯作者：
Cooper, Jonathan M.
中科院分区：
文献类型：
--
作者：
Bourquin, Yannyk;Reboud, Julien;Cooper, Jonathan M.
The diagnosis of infectious diseases in the Developing World is technologically challenging requiring complex biological assays with a high analytical performance, at minimal cost. By using an opto-acoustic immunoassay technology, integrating components commonly used in mobile phone technologies, including surface acoustic wave (SAW) transducers to provide pressure driven flow and a CMOS camera to enable lensfree detection technique, we demonstrate the potential to produce such an assay. To achieve this, antibody functionalised microparticles were manipulated on a low-cost disposable cartridge using the surface acoustic waves and were then detected optically. Our results show that the biomarker, interferon-gamma, used for the diagnosis of diseases such as latent tuberculosis, can be detected at pM concentrations, within a few minutes (giving high sensitivity at a minimal cost).
