MicroRNAs are Critical Regulators of Osteoclast Differentiation.
MicroRNAs are Critical Regulators of Osteoclast Differentiation.
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DOI：
10.1007/s40610-019-0116-3
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发表时间：
2019-02-01
期刊：
影响因子：
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通讯作者：
Delany, Anne M
中科院分区：
文献类型：
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作者：
Hrdlicka, Henry C;Lee, Sun-Kyeong;Delany, Anne M
综述目的：我们的目标是全面回顾最新报道的microRNA（miRNA）调控破骨细胞生成。我们强调验证的miRNA-靶相互作用及其在控制破骨细胞分化和function.Recent发现的信号网络中的位置：使用无偏的方法来确定感兴趣的miRNA和报告基因-3 'UTR检测来验证相互作用，最近的研究已经阐明了特定的miRNA-mRNA相互作用在体外破骨细胞生成过程中的影响。一直关注RANK和CSF 1 R信号传导下游的信号传导介质，以及分化和功能所必需的基因。例如，几种miRNAs直接或间接靶向破骨细胞主转录因子Nfatc 1（例如miR-124和miR-214）和Rho-GTP酶、Cdc 42和Rac 1（例如miR-29家族）。验证miRNA表达模式、靶标，和影响破骨细胞和其他骨骼细胞是至关重要的理解基本骨生物学和实现治疗潜力的miRNA-治疗骨病的基本策略。
PURPOSE OF REVIEW: Our goal is to comprehensively review the most recent reports of microRNA (miRNA) regulation of osteoclastogenesis. We highlight validated miRNA-target interactions and their place in the signaling networks controlling osteoclast differentiation and function.RECENT FINDINGS: Using unbiased approaches to identify miRNAs of interest and reporter-3'UTR assays to validate interactions, recent studies have elucidated the impact of specific miRNA-mRNA interactions during in vitro osteoclastogenesis. There has been a focus on signaling mediators downstream of the RANK and CSF1R signaling, and genes essential for differentiation and function. For example, several miRNAs directly and indirectly target the master osteoclast transcription factor, Nfatc1 (e.g. miR-124 and miR-214) and Rho-GTPases, Cdc42 and Rac1 (e.g. miR-29 family).SUMMARY: Validating miRNA expression patterns, targets, and impact in osteoclasts and other skeletal cells is critical for understanding basic bone biology and for fulfilling the therapeutic potential of miRNA-based strategies in the treatment bone diseases.