SYNTHESIS AND CHARACTERIZATION OF COLLOIDAL DISPERSIONS OF FLUORESCENT, MONODISPERSE SILICA SPHERES
SYNTHESIS AND CHARACTERIZATION OF COLLOIDAL DISPERSIONS OF FLUORESCENT, MONODISPERSE SILICA SPHERES
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DOI：
10.1021/la00048a013
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发表时间：
1992-12-01
期刊：
影响因子：
3.9
通讯作者：
VRIJ, A
中科院分区：
文献类型：
--
作者：
VANBLAADEREN, A;VRIJ, A
用一般方法合成了含有染料或荧光团的单分散胶体二氧化硅微球的稳定分散体，并将其分散在极性和非极性液体中。该过程包括将染料偶联到硅烷偶联剂(3-氨丙基)三乙氧基硅烷，并将反应产物可控地结合到二氧化硅球体中。硅球是由正硅酸乙酯在氨、水和乙醇的混合物中制备的。二氧化硅球的组成可以这样控制，使得有机基团可以放置在表面上，放置在颗粒内的薄壳中，或者通过内核的体积分布。异硫氰酸荧光素被用来制造易于漂白的荧光二氧化硅微球。合成了亲水、电荷稳定和有机空间稳定的1-十八醇包覆染料二氧化硅体系，并将其分散在多种溶剂中。经过几个反应步骤后，用静态和动态光散射和透射电子显微镜对所制备的粒子进行了表征。利用荧光光谱和激光共聚焦扫描荧光显微镜对所制备的荧光微球进行了表征。为了制备没有颗粒团的单分散分散体，人们付出了很大的努力。这种模型色散是(散射)研究(浓缩)系统中粒子间相互作用所必需的。因此，对合成和光学表征的几个步骤进行了详细的描述。
Stable dispersions of monodisperse colloidal silica spheres containing a dye or fluorophore have been synthesized according to a general procedure and dispersed in polar and apolar liquids. The procedure consists of the coupling of the dye to a silane coupling agent, (3-aminopropyl)triethoxysilane, and the controllable incorporation of the reaction product into the silica sphere. The silica spheres are prepared from tetraethoxysilane in mixtures of ammonia, water, and ethanol. The composition of the silica spheres can be controlled in such a way that the organic groups can be placed on the surface, in a thin shell inside the particle or distributed through the volume of an inner core. Fluorescein isothiocyanate was used to make easily bleachable, fluorescent silica spheres. Hydrophilic charge stabilized and organophilic sterically stabilized 1-octadecanol-coated dyed silica systems were synthesized and dispersed in several solvents. All the particles were characterized after the several reaction steps by static and dynamic light scattering and transmission electron microscopy. The fluorescent spheres were further characterized by fluorescence spectroscopy and confocal scanning laser fluorescence microscopy. Great effort was taken to prepare monodisperse dispersions free of clusters of particles. Such model dispersions are required for (scattering) studies of interparticle interactions in (concentrated) systems. Therefore, the several steps of the synthesis and optical characterization are described in detail.