Protein accumulation in lung lavage fluid following ozone exposure.
Protein accumulation in lung lavage fluid following ozone exposure.
复制标题
臭氧暴露后肺灌洗液中蛋白质积聚。
DOI：
10.1016/0013-9351(82)90039-1
复制
发表时间：
1982
影响因子：
8.3
通讯作者：
M. Selgrade
中科院分区：
文献类型：
--
作者：
Ping C. Hu;Frederick J. Miller;Mary J. Daniels;Gary E. Hatch;Judith A. Graham;Donald E. Gardner;M. Selgrade
Accumulation of protein in lung lavage fluid was used as an indicator of pulmonary damage following exposure of guinea pigs to O3. Exposure of animals to 510, 1000, or 1960 μg/m3(0.26, 0.51, or 1.0 ppm) of O3for 72 hr resulted in significantly elevated levels of lavage fluid protein when compared to that of air controls. This effect was not observed in animals exposed to 196 μg O3/m3(0.10 ppm). When exposure time was reduced to 3 hr, the O3-induced protein accumulation in lavage fluids was undetectable unless the time of lavage was delayed 10–15 hr following the exposure. Under these conditions, elevated protein content was seen in lung lavage fluids obtained from animals exposed to O3ranging from 510 to 1470 μg O3/m3(0.26-0.75 ppm) and a dose relationship between the amount of protein accumulation in the lung and the concentration of O3to which the animals were exposed was observed. Vitamin C deficiency did not enhance this O3-induced lesion in guinea pigs. The dose relationship has also been confirmed by polyacrylamide gel electrophoresis of the lavage fluids. Lung lavage fluid protein content in animals exposed to 353 μg O3/m3(0.18 ppm) for 8 hr/day for 5 or 10 consecutive days was not different from that of air controls.
