Molecular characterization of prothoracicotropic hormone and diapause hormone in Heliothis virescens during diapause, and a new role for diapause hormone
Molecular characterization of prothoracicotropic hormone and diapause hormone in Heliothis virescens during diapause, and a new role for diapause hormone
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DOI：
10.1046/j.1365-2583.2003.00437.x
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发表时间：
2003-10-01
影响因子：
2.6
通讯作者：
Denlinger, DL
中科院分区：
文献类型：
--
作者：
Xu, WH;Denlinger, DL
从烟青虫Heliothis virescens（Hvi）中分离出编码促前胸激素（PTTH）和滞育激素（DH）的cDNA。Hvi-PTTH cDNA揭示了从其他PTTH已知的关键结构特征，然而当与从海蛞蝓总科已知的PTTH相比时，在该海蛞蝓中发现的PTTH序列之间存在> 30%的氨基酸差异。Hvi-DH-PBAN cDNA编码包括DH样肽、信息素生物合成激活神经肽（PBAN）和在C末端具有FXPRL序列的另外三种神经肽的前体蛋白。PTTH和DH-PBAN转录物分别在脑和食管下神经节（SG）中表达最高。PTTH和DH-PBAN转录的表达是高的幼虫，并保持在nondiapausing蛹高，但在幼虫编程蛹滞育，表达急剧下降，在发病的幼虫游走行为，并保持在蛹滞育低。这种模式意味着这两个基因在滞育期间都被关闭。这些结果是不一致的作用，DH在促进进入滞育注意到在家蚕。相反，DH在非滞育蛹中的较高表达表明DH在促进持续发育中可能起作用。将DH注射到滞育蛹中确实成功地终止了滞育，从而表明这种神经肽可能具有新的作用。
cDNAs encoding prothoracicotropic hormone ( PTTH) and diapause hormone (DH) were isolated from the tobacco budworm, Heliothis virescens (Hvi). Hvi-PTTH cDNA reveals key structural features known from other PTTHs, yet there is a > 30% amino acid difference between the PTTH sequences found in this noctuid when compared with the PTTHs known from Bombycoidea. Hvi-DH-PBAN cDNA encodes a precursor protein including a DH-like peptide, pheromone biosynthesis-activating neuropeptide ( PBAN) and three additional neuropeptides with an FXPRL sequence at the C terminus. PTTH and DH-PBAN transcripts are most highly expressed in the brain and subesophageal ganglion (SG), respectively. Expression of both the PTTH and the DH-PBAN transcripts is high in larvae and remains high in nondiapausing pupae, but in larvae programmed for pupal diapause, expression declines sharply at the onset of larval wandering behaviour and remains low during pupal diapause. This pattern implies that both of these genes are shut down during diapause. These results are not consistent with a role for DH in promoting the entry into diapause as noted in Bombyx mori. Instead, the higher expression of DH in nondiapausing pupae suggests a possible role for DH in promoting continuous development. The injection of DH into diapausing pupae did indeed successfully terminate diapause, thus suggesting a possible new role for this neuropeptide.