Algicidal activity and gilding motility of Saprospira sp SS98-5
Algicidal activity and gilding motility of Saprospira sp SS98-5
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DOI：
10.1139/w03-017
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发表时间：
2003-02-01
影响因子：
2.8
通讯作者：
Sakata, T
中科院分区：
文献类型：
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作者：
Furusawa, G;Yoshikawa, T;Sakata, T
从日本鹿儿岛湾分离出的一种海洋细菌Saprospira sp. SS98-5能够杀死并溶解硅藻角孢毛藻（Chaetoceros ceratosporum）的细胞。这种细菌的多细胞丝状细胞捕获硅藻细胞，形成细胞聚集体，并在富集的海水（ESS）液体培养基中裂解它们。菌株SS98-5也在含有硅藻细胞的双层琼脂板上形成斑块。硅藻细胞壁在与细菌接触的部位被部分降解，细菌从那里侵入硅藻细胞，然后硅藻细胞被完全裂解。菌株具有滑动运动能力，在含有低浓度多蛋白胨（0.1%以下）的ESS琼脂板上生长为扩散菌落，而在含有0.5%多蛋白胨的ESS琼脂板上形成非扩散菌落。电镜超薄切片显示，微管样结构仅在滑动运动细胞中可见。在添加足臼毒素（一种微管组装抑制剂）后，细菌细胞的滑行动力和微管样结构都减弱了，这表明在这些细菌细胞中观察到的微管样结构与它们的滑行动力有关。
A marine bacterium, Saprospira sp. SS98-5, which was isolated from Kagoshima Bay, Japan, was able to kill and lyse the cells of the diatom Chaetoceros ceratosporum. The multicellular filamentous cells of this bacterium captured the diatom cells, formed cell aggregates, and lysed them in an enriched sea water (ESS) liquid medium. Strain SS98-5 also formed plaques on double layer agar plates incorporating diatom cells. The diatom cell walls were partially degraded at the contact sites with the bacteria, the bacteria invaded from there into the diatom cells, and then the diatom cells were completely lysed. The strain possessed gliding motility and grew as spreading colonies on ESS agar plates containing lower concentrations of polypeptone (below 0.1%) while forming nonspreading colonies on ESS agar plates containing 0.5% polypeptone. Electron micrographs of ultrathin sections demonstrated that microtubule-like structures were observable only in gliding motile cells. Both the gliding motility and the micrombule-like structures were diminished by the addition of podophyllotoxin, an inhibitor of microtubule assembly, suggesting that the microtubule-like structures observed in these bacterial cells are related to their gliding motility.