Newly Emerged Porcine Deltacoronavirus Associated With Diarrhoea in Swine in China: Identification, Prevalence and Full-Length Genome Sequence Analysis
Newly Emerged Porcine Deltacoronavirus Associated With Diarrhoea in Swine in China: Identification, Prevalence and Full-Length Genome Sequence Analysis
复制标题
与中国猪腹泻相关的新出现的猪三角洲冠状病毒：鉴定、流行率和全长基因组序列分析
DOI：
10.1111/tbed.12399
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发表时间：
2015-12-01
影响因子：
4.3
通讯作者：
Tang, Y.
中科院分区：
文献类型：
--
作者：
Song, D.;Zhou, X.;Tang, Y.
为了鉴定和表征与2015年3月中国江西省仔猪严重腹泻爆发相关的病原体，开发了用于检测猪三角洲冠状病毒（PDCoV）的巢式逆转录-聚合酶链反应（RT-PCR）。应用建立的巢式RT-PCR方法，对江西省部分地区猪群进行了PDCoV单感染（33.71%）和PDCoV与猪流行性腹泻病毒（PEDV）混合感染（19.66%）的调查。在PEDV阴性的粪便样品中观察到PDCoV的高患病率（58.33%）。测定了代表性PDCoV毒株PDCoV/CHJXNI 2/2015的全基因组序列。对PDCoV/CHJXNI 2/2015的全基因组和S蛋白序列进行系统发育分析，结果表明该毒株与香港和美国的PDCoV关系最密切。据我们所知，这是第一个报告的鉴定，流行，全基因组测序和分子特征的PDCoV在中国的猪腹泻样品。
To identify and characterize aetiologic agent(s) associated with an outbreak of a severe diarrhoea in piglets in Jiangxi, China, in March 2015, a nested reverse transcription-polymerase chain reaction (RT-PCR) for the detection of porcine deltacoronavirus (PDCoV) was developed. A survey based on the nested RT-PCR established indicated that the monoinfection of PDCoV (33.71%) and coinfection of PDCoV (19.66%) with porcine epidemic diarrhoea virus (PEDV) were common in diarrhoeal pigs in Jiangxi, China. A high prevalence of PDCoV (58.33%) in diarrhoeal samples which were PEDV negative was observed. The complete genome sequence of a representative PDCoV strain, PDCoV/CHJXNI2/2015, was determined. Phylogenetic analysis of complete genome and S protein sequences of PDCoV/CHJXNI2/2015 demonstrated that it was most closely related to Hong Kong and US PDCoVs. To our knowledge, this is the first report on the identification, prevalence, complete genome sequencing and molecular characterizations of PDCoV in diarrhoeal samples in pigs in China.