Plant-plant interactions and N fertilization shape soil bacterial and fungal communities
Plant-plant interactions and N fertilization shape soil bacterial and fungal communities
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DOI：
10.1016/j.soilbio.2018.10.018
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发表时间：
2019-01-01
影响因子：
9.7
通讯作者：
Li, Chunyang
中科院分区：
文献类型：
--
作者：
Guo, Qingxue;Yan, Lijuan;Li, Chunyang
在森林生态系统中，同种和异种相邻植物对土壤细菌和真菌群落的影响从未被探索过。本研究首先对日本落叶松人工林、日本落叶松人工林和落叶松人工林的土壤微生物群落进行了研究。olgensis单作及其混作。在此基础上，通过两年的生长试验，研究了种内和种间互作效应。kaempferi和L.两种氮素水平下，olgensis对根际微生物群落的影响结果表明，3种人工林土壤细菌和真菌的β多样性和组成存在明显差异，表明植物-植物相互作用对土壤微生物群落的影响存在差异。盆栽试验结果表明，L.无论施氮与否，山奈的邻体效应均大于其同种邻体效应，但施氮后山奈的邻体效应减弱。将山奈与L.施氮条件下，种内和种间互作的变化显著影响了施氮条件下土壤的化学和生物学性质，在种内互作条件下，L. kaempferi（KK）的种间相互作用。kaempferi和L. olgensis（KO）。施氮增加了KO根际土壤中细菌和真菌的α-二氢叶酸。对于β多样性，PERMANOVA结果表明，种内和种间植物相互作用对土壤微生物群落有显著影响，KK与L. olgensis（OO）和KO.这两种植物和氮肥对土壤微生物组成，特别是真菌群落表现出特定的影响。两个湖在KO + N条件下，子囊菌门的丰度增加，而担子菌门的丰度降低，甚至优势菌由担子菌门向子囊菌门转移。
The impact of conspecific and heterospecific neighboring plants on soil bacterial and fungal communities has never been explored in a forest ecosystem. In the present study, we first investigated soil microbial communities in three plantations: Larix kaempferi monoculture, L. olgensis monoculture and their mixture. Then, a two-year growth experiment was conducted to investigate the effects of intra- and inter-specific interactions of L. kaempferi and L. olgensis on rhizosphere microbial communities at two different nitrogen levels. The results demonstrated clear differences in the beta-diversity and composition of bacteria and fungi among the three plantations, which implied the presence of different effects of plant-plant interactions on soil microbial communities. The results of the pot experiment showed that L. kaempferi suffered from greater neighbor effects from its conspecific neighbor regardless of N fertilization, although the effect declined when L. kaempferi was grown with L. olgensis under N fertilization. Changes in intra- and inter-specific plant interactions significantly impacted the chemical and biological properties of soil under N fertilization, with lower concentrations of NH4+, and lower soil microbial biomass (C-Mic) and soil carbon nitrogen biomass (N-Mic) under intra-specific plant interactions of L. kaempferi (KK) compared to inter-specific interactions of L. kaempferi and L. olgensis (KO). N fertilization increased bacterial and fungal alpha diversities in the rhizosphere soil of KO. For the beta diversity, the PERMANOVA results demonstrated that there was a significant impact of intra- and inter-specific plant interactions on soil microbial communities, with KK significantly differing from intra-specific plant interactions of L. olgensis (OO) and KO. The two plant species and N fertilization showed specific effects on the soil microbial composition, particularly on the fungal community. Both L. olgensis and N fertilization increased the abundance of Ascomycota but reduced that of Basidiomycota, and even shifted the dominance from Basidiomycota to Ascomycota under KO combined with N fertilization.