Rapid and accurate identification of in vivo-induced haploid seeds based on oil content in maize
Rapid and accurate identification of in vivo-induced haploid seeds based on oil content in maize
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DOI：
10.1038/srep02129
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发表时间：
2013-07-03
影响因子：
4.6
通讯作者：
Technow, Frank
中科院分区：
文献类型：
--
作者：
Melchinger, Albrecht E.;Schipprack, Wolfgang;Technow, Frank
不断增长的人口需求要求植物育种家快速有效地开发改良品种。双单倍体（DH）技术可以在一个步骤中产生完全纯合子系，因此是现代遗传学和育种方法的核心。在玉米体内单倍体诱导后快速、可靠地鉴定具有单倍体胚的种子是一种基本方法，但目前的系统存在严重缺陷，阻碍了其在许多种质类型中的应用。在这里，我们描述了一种基于高含油量诱导剂授粉产生的单倍体和二倍体种子含油量差异来区分单倍体和二倍体种子的方法。在介绍了一些基本理论之后，我们用实验结果提供了概念验证，展示了不同种质的可接受错误率。我们的方法代表了玉米DH技术的突破，因为它适合于自动化高通量筛选，并且适用于全球任何玉米种质。
The needs of a growing human population require rapid and efficient development of improved cultivars by plant breeders. The doubled haploid (DH) technology enables generating completely homozygous lines in a single step and, thus, is central to modern genetics and breeding approaches. Rapid and reliable identification of seeds with a haploid embryo after in vivo haploid induction is elementary in the method utilized in maize but current systems have severe shortcomings preventing their use in many germplasm types. Here, we describe an alternative method for discrimination of haploid from diploid seeds based on differences in their oil content stemming from pollination with high oil inducers. After presenting some fundamental theory, we provide a proof-of-concept with experimental results, demonstrating acceptable error rates across different germplasm. Our approach represents a breakthrough in DH technology in maize, because it is amenable to automated high-throughput screening and applicable to any maize germplasm worldwide.