INTRAMURAL NEURONS OF THE GUINEA-PIG URINARY-BLADDER - HISTOCHEMICAL-LOCALIZATION OF PUTATIVE NEUROTRANSMITTERS IN CULTURES AND NEWBORN ANIMALS
INTRAMURAL NEURONS OF THE GUINEA-PIG URINARY-BLADDER - HISTOCHEMICAL-LOCALIZATION OF PUTATIVE NEUROTRANSMITTERS IN CULTURES AND NEWBORN ANIMALS
复制标题
DOI：
10.1016/0165-1838(86)90018-4
复制
发表时间：
1986-04-01
期刊：
影响因子：
--
通讯作者：
BURNSTOCK, G
中科院分区：
文献类型：
--
作者：
CROWE, R;HAVEN, AJ;BURNSTOCK, G
用组织化学方法研究了新生豚鼠膀胱和腹壁神经节培养物中神经递质的分布。根据烟酰胺腺嘌呤二核苷酸(NADH)-脱氢酶反应特异性标记神经细胞体，在新生儿膀胱拉伸准备中观察到多达66个神经节。每个神经节包含2-50个神经细胞体。血管活性肠多肽在原位和培养制剂中均定位于部分神经节内神经细胞体。在原位制备中，它广泛分布于神经纤维到肌肉中，在膀胱底部和一些粘膜上皮细胞中密度最大。生长抑素存在于逼尿肌的许多神经元细胞体中，无论是原位的还是培养的。广泛分布的静脉曲张纤维被发现在培养和肌肉，粘膜下和粘膜层原位。P物质免疫荧光在神经节的少数神经元胞体中均可见，特别是在膀胱底部的粘膜中。在原位制剂中，含有P物质的静脉曲张神经纤维可见于膀胱基部密度最大的肌层。met -脑啡肽免疫反应性神经细胞体在原位和培养中均未见。原位制备的神经纤维大部分包裹着神经节内的神经元细胞体。原位膀胱制备中既未见血清素免疫反应性神经元细胞体，也未见含儿茶酚胺神经元细胞体。然而，培养中的一些神经细胞体显示阳性染色，可能是由于培养基中已知的胎牛血清中含有的5 -羟色胺和儿茶酚胺的选择性摄取，也可能是由于培养情况下某些神经元的合成活性增加。在全贴装拉伸制剂中，未见血清素免疫反应神经纤维，但观察到含有儿茶酚胺的小的强烈荧光细胞和神经纤维。膀胱原位和培养标本中均可见乙酰胆碱酯酶阳性的神经细胞体和神经纤维。在原位检查的许多胞内神经元中发现了喹那昔宁阳性的神经细胞体（作为嘌呤能神经元的指示物）；然而，在所使用的培养条件下，所有细胞类型都发生非选择性染色。
Histochemical methods have been used to study the distribution of putative neurotransmitters in the urinary bladder of newborn guinea-pigs and in cultures of intramural ganglia. Following the nicotinamide adenine dinucleotide (NADH)-diaphorase reaction which specifically labels nerve cell bodies, up to 66 ganglia were observed in stretch preparations of the newborn urinary bladder. Each ganglion contained 2-50 nerve cell bodies. Vasoactive intestinal polypeptide was localized in a few nerve cell bodies of intramural ganglia both in in situ and culture preparations. In the in situ preparations it was widely distributed in nerve fibres to the muscle, being most dense at the base of the bladder, and in some mucosal epithelial cells. Somatostatin was contained in numerous neuronal cell bodies in the detrusor muscle both in situ and in culture. Extensively distributed varicose fibres were found in culture and in the muscle, submucous and mucosal layers in situ. Substance P immunofluorescence was demonstrated in a few neuronal cell bodies in ganglia both in situ and in vitro, particularly in those of the mucosa at the base of the bladder. In the in situ preparations varicose nerve fibres containing substance P were seen in the muscle coats with greatest density in the bladder base. Met-enkephalin-immunoreactive nerve cell bodies were not seen either in situ or in culture. Nerve fibres in in situ preparations were found largely enveloping neuronal cell bodies within the ganglia. Neither serotonin-immunoreactive nor catecholamine-containing neuronal cell bodies were seen in the in situ bladder preparation. However, some nerve cell bodies in culture showed positive staining, possibly as a result of selective uptake of serotonin and catecholamine known to be contained in foetal calf serum in the culture medium or possibly as the result of increased synthetic activity in certain neurones in the culture situation. In whole-mount stretch preparations, no serotonin-immunoreactive nerve fibres were seen, but catecholamine-containing small intensely fluorescent cells and nerve fibres were observed. Acetylcholinesterase-positive nerve cell bodies and nerves fibres were observed both in in situ and culture preparations of the bladder. Quinacrine-positive nerve cell bodies (as an indicator of purinergic neurones) were found in numerous intramural neurones examined in situ; however, under the culture conditions used, non-selective staining of all cell types occurred.