Characterization of Glycoside Hydrolase Family 6 Enzymes from Coprinopsis cinerea
Characterization of Glycoside Hydrolase Family 6 Enzymes from Coprinopsis cinerea
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灰鬼伞 (Coprinopsis cinerea) 糖苷水解酶家族 6 酶的表征
DOI：
10.1271/bbb.80888
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发表时间：
2009
期刊：
影响因子：
--
通讯作者：
Makoto Yoshida
中科院分区：
文献类型：
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作者：
Yuan Liu;K. Igarashi;S. Kaneko;T. Tonozuka;M. Samejima;K. Fukuda;Makoto Yoshida
来自灰鬼伞 (Coprinopsis cinerea) 的重组 Cel6 (CcCel6)A、-B 和 -C 由大肠杆菌表达。这些酶从磷酸溶胀的纤维素中产生纤维二糖。当使用Avicel作为底物时，CcCel6A对底物表现出比CcCel6B或-C更高的活性。在与羧甲基纤维素反应时，CcCel6B 和-C 水解底物，而 CcCel6A 未能反应。
Recombinants, Cel6 from Coprinopsis cinerea (CcCel6)A, -B, and -C, were expressed by Escherichia coli. These enzymes produced cellobiose from phosphoric acid-swollen cellulose. When Avicel was used as the substrate, CcCel6A showed higher activity toward the substrate than CcCel6B or -C. In reaction with carboxymethylcellulose, CcCel6B and -C hydrolyzed the substrate, whereas CcCel6A failed to react.