Single‐worm quantitative proteomics reveals aging heterogeneity in isogenic Caenorhabditis elegans
Single‐worm quantitative proteomics reveals aging heterogeneity in isogenic Caenorhabditis elegans
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DOI：
10.1111/acel.14055
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发表时间：
2023-12
期刊：
影响因子：
7.8
通讯作者：
Tian-Yi Zhu;Shang-Tong Li;Dan-Dan Liu-Dan;Xiajun Zhang;Lianqi Zhou;Rong Zhou;Bing Yang
中科院分区：
文献类型：
--
作者：
Tian-Yi Zhu;Shang-Tong Li;Dan-Dan Liu-Dan;Xiajun Zhang;Lianqi Zhou;Rong Zhou;Bing Yang
在小鼠模型和人类中，已经在细胞和器官水平上研究了衰老的异质性，但缺乏在整个生物体水平上对衰老异质性的探索。C.秀丽线虫是研究这个问题的理想模式生物，因为它们是在同一个腔室中自我受精和培养的。尽管单细胞蛋白质组学分析取得了巨大进展，但关于衰老的单虫蛋白质组学研究很少。在这里，我们应用单蠕虫定量质谱来量化个体衰老过程中的异质性蛋白质组学变化，来自157个C的总共3524个蛋白质。对大象个体进行定量。重建的C。elegans老化轨迹和快速老化个体的蛋白质组景观用于分析C. elegans老化我们描述了衰老过程中个体间蛋白质组的变化，并揭示了将快速衰老个体与其兄弟姐妹区分开来的因素。
The heterogeneity of aging has been investigated at cellular and organic levels in the mouse model and human, but the exploration of aging heterogeneity at whole‐organism level is lacking. C. elegans is an ideal model organism for studying this question as they are self‐fertilized and cultured in the same chamber. Despite the tremendous progress made in single‐cell proteomic analysis, there is few single‐worm proteomics studies about aging. Here, we apply single‐worm quantitative mass spectrometry to quantify the heterogenous proteomic changes during aging across individuals, a total of 3524 proteins from 157 C. eleagns individuals were quantified. A reconstructed C. elegans aging trajectory and proteomic landscape of fast‐aging individuals were used to analyze the heterogeneity of C. elegans aging. We characterized inter‐individual proteomic variation during aging and revealed contributing factors that distinguish fast‐aging individuals from their siblings.