Characterization of faecal microbial communities of dairy cows fed diets containing ensiled Moringa oleifera fodder.
Characterization of faecal microbial communities of dairy cows fed diets containing ensiled Moringa oleifera fodder.
复制标题
饲喂含有青贮辣木饲料的奶牛粪便微生物群落的特征
DOI：
10.1038/srep41403
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发表时间：
2017-01-30
影响因子：
4.6
通讯作者：
Zhang Y
中科院分区：
文献类型：
--
作者：
Sun J;Zeng B;Chen Z;Yan S;Huang W;Sun B;He Q;Chen X;Chen T;Jiang Q;Xi Q;Zhang Y
辣木(Moringa oleifera，M.oleifera)是一种营养价值高、生物量大的优良牧草，可作为家畜饲料。在这项研究中，我们研究了30头奶牛粪便微生物区系的变化，以及它们对营养消化、牛奶特性和粪便短链脂肪酸浓度的影响。在对照和油松根霉替代组之间没有发现产奶量和乳汁成分的差异。饲喂油菜青贮组奶牛干物质消化率、丙酸和异戊酸浓度均低于对照组。利用16S rDNA基因测序，获得了1,299,556个成对末端读数。聚类分析表明，所有样本均隶属于13门93属。浮游植物和拟杆菌属是共优势门。10个类群对高油菜摄食的反应有显著差异。此外，阿克曼氏菌和普雷沃特氏菌与产奶量和蛋白质的相关性很强，表明某些细菌群可用于改良牛奶品质。我们的结果提供了一个深入了解微生物组相关的反应在家畜饲料中，并可能有助于开发新的应用。
Moringa oleifera (M. oleifera) is a remarkable species with high nutritional value and good biomass production, which can be used as livestock fodder. In this study, we examined changes in the faecal microbiota of thirty dairy cows in response to alternative M. oleifera diets and their effects on nutrient digestion, milk traits and the faecal concentrations of short-chain fatty acids. No differences in milk yield and constituents were found between the control and the M. oleifera alternative groups. Cows fed M. oleifera silage had lower dry matter digestibility, as well as the propionate and isovalerate concentrations in M. oleifera treated group. Using 16S rDNA gene sequencing, 1,299,556 paired-end reads were obtained. Clustering analysis revealed 13 phyla and 93 genera across all samples. Firmicutes and Bacteroidetes were the co-dominant phyla. Ten taxa displayed a significant difference in response to the high M. oleifera diet. In addition, strong correlations between Akkermansia and Prevotella with milk yield and protein indicated that some bacterial groups could be used to improve milk traits. Our results provided an insight into the microbiome-associated responses to M. oleifera in livestock diets, and could aid the development of novel applications of M. oleifera.