Expression of calpastatin and myostatin genes associated with lamb meat quality
Expression of calpastatin and myostatin genes associated with lamb meat quality
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DOI：
10.4238/2013.december.4.3
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发表时间：
2013-01-01
影响因子：
0.4
通讯作者：
Del Vesco, A. P.
中科院分区：
文献类型：
--
作者：
Bagatoli, A.;Gasparino, E.;Del Vesco, A. P.
钙调蛋白(CAST)是一种内源性钙蛋白酶抑制剂，其主要功能是调节导致死后肌原纤维恶化的酶的蛋白水解性作用。肌肉生长抑制素基因(GDF-8)是骨骼肌生长的负调控因子。这两个基因的表达及其相互作用影响着肉品的品质，尤其是嫩度表型。以2.0 mm、2.5 mm和3.0 mm脂肪厚度屠宰的圣诞老人、1/2Dorper-Santa Ines和白色Dorper-Santa Ines为研究对象，比较了CAST和GDF-8基因的表达水平与体重性能和屠体性状，特别是剪切力值的关系。我们发现，在Santa Ines遗传组中，myostatin和calastatin的表达明显较高。当屠宰脂肪厚度为2.0毫米和2.5毫米时，多珀-圣伊内斯遗传组这些基因的表达最低。综上所述，Santa Ines品种的肉嫩度表型值最低，而Dorper-Santa Ines品种的这一特性表现最好。我们认为，CAST和GDF-8基因的高水平表达与羊肉嫩度的低值有关，嫩度与肌肉的生长发育阶段有关。
Calpastatin (CAST) is an endogenous calpain inhibitor and its main function is to modulate the proteolytic action of enzymes responsible for post-mortem myofibril deterioration. The myostatin gene (GDF-8) acts as a negative regulator of skeletal muscle growth. The expression of these two genes, as well as their interaction, affects the quality of the meat, especially the tenderness phenotype. We evaluated the genetic groups Santa Ines, 1/2 Dorper-Santa Ines and White Dorper-Santa Ines, slaughtered with 2.0 mm, 2.5 mm and 3.0 mm of fat thickness, comparing the levels of expression of the CAST and GDF-8 genes with the weight performance and carcass traits, especially the shear force values. We found significantly higher expression of myostatin and calpastatin in the Santa Ines genetic group. The Dorper-Santa Ines genetic group had the lowest expression of these genes when slaughtered with 2.0 and 2.5 mm of fat thickness. In conclusion, the Santa Ines breed had the lowest phenotype values for meat tenderness, and the Dorper-Santa Ines breed had the best performance for this characteristic. We suggest that high levels of the expression of the CAST and GDF-8 genes are associated with lower values of lamb meat tenderness, and that tenderness is related to the stage of muscular growth and development.