ACTION OF PBAN AND RELATED PEPTIDES ON PHEROMONE BIOSYNTHESIS IN ISOLATED PHEROMONE GLANDS OF THE REDBANDED LEAFROLLER MOTH, ARGYROTAENIA-VELUTINANA
ACTION OF PBAN AND RELATED PEPTIDES ON PHEROMONE BIOSYNTHESIS IN ISOLATED PHEROMONE GLANDS OF THE REDBANDED LEAFROLLER MOTH, ARGYROTAENIA-VELUTINANA
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DOI：
10.1016/1367-8280(94)90026-4
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发表时间：
1994-07-01
期刊：
影响因子：
--
通讯作者：
ROELOFS, WL
中科院分区：
文献类型：
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作者：
JURENKA, RA;FABRIAS, G;ROELOFS, WL
以红带卷叶蛾信息素腺体为材料，研究了信息素生物合成激活神经肽(PBAN)、法氏囊信息素促性肽及其他几种相关多肽对信息素生物合成的影响。通过信息素滴度和掺入放射性标记的乙酸酯，所有属于PBAN家族的多肽和法氏囊多肽都能刺激信息素的生物合成。这些多肽需要细胞外钙离子存在才能表达全部活性，而几种无机钙通道阻断剂抑制信息素生物合成的刺激。钙离子载体A23187单独刺激信息素的生物合成，cAMP类似物也是如此。观察cAMP类似物在无细胞外钙条件下的刺激作用。在16小时的腺体孵化中观察到信息素的最大效价；然而，如果通过掺入放射性标记的醋酸酯来测量信息素的生物合成，则需要2-6小时的孵育。在PBAN存在的情况下，放射性标记的葡萄糖掺入没有增加。这些结果是在信息素生物合成途径如何被这些多肽刺激的背景下讨论的。
Isolated pheromone glands from the redbanded leafroller moth, Argyrotaenia velutinana, were utilized to demonstrate the action of pheromone biosynthesis activating neuropeptide (PBAN) and bursa pheromonotropic peptide plus several other related peptides on pheromone biosynthesis. All peptides belonging to the PBAN family and the bursa peptide stimulated pheromone biosynthesis as measured by pheromone titer and incorporation of radiolabeled acetate.These peptides required the presence of extracellular Ca2+ for expression of full activity and several inorganic Ca2+ channel blockers inhibited the stimulation of pheromone biosynthesis. The Ca2+ ionophore A23187 alone stimulated pheromone biosynthesis as did a cAMP analogue. Stimulation by the cAMP analogue in the absence of extracellular Ca2+ was observed. Maximum pheromone titers were observed in 16 hr gland incubations; however, 2-6 hr incubations were required if pheromone biosynthesis was measured by incorporation of radiolabeled acetate. Radiolabeled glucose incorporation was not increased in the presence of PBAN. These results are discussed in the context of how the pheromone biosynthetic pathway is stimulated by these peptides.