Multiphoton intrapulse interference 3: Probing microscopic chemical environments
Multiphoton intrapulse interference 3: Probing microscopic chemical environments
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DOI：
10.1021/jp036150o
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发表时间：
2004-01-08
影响因子：
2.9
通讯作者：
Dantus, M
中科院分区：
文献类型：
--
作者：
Dela Cruz, JM;Pastirk, I;Dantus, M
相位调制通过其对特定频率下双光子激发概率的影响，被用来探测分子的微观化学环境。根据多光子脉冲内干涉原理设计所需的光谱相位。我们展示了一个实验结果，其中相位调制的20秒脉冲从钛蓝宝石激光振荡器控制双光子诱导荧光的强度从ph敏感染料8-羟基芘-1,3,6-三磺酸（HPTS）在溶液中。我们表明，荧光产率对相的依赖可以用作区分不同环境中的分子的手段。因此，这种方法可以用来实现选择性多光子显微镜。为了说明这种能力，我们展示了相位调制用于选择性地激发酸性或碱性微观区域的HPTS的图像。
Phase modulation, through its influence on the probability of two-photon excitation at specific frequencies, is used to probe a molecule's microscopic chemical environment. The spectral phase required is designed according to the principles of multiphoton intrapulse interference. We show experimental results where phase modulation of 20 fs pulses from a titanium sapphire laser oscillator controls the intensity of two-photon induced fluorescence from the pH-sensitive dye 8-hydroxypyrene-1,3,6-trisulfonic acid (HPTS) in solution. We show that the dependence of fluorescence yield on the phase can be utilized as a means of distinguishing molecules in different environments. Consequently, this method can be used to achieve selective multiphoton microscopy. To illustrate this capability, we show images where phase modulation was used to selectively excite HPTS in acidic or basic microscopic regions.