In situ examination of the time-course for secondary mineralization of Haversian bone using synchrotron Fourier transform infrared micro spectroscopy
In situ examination of the time-course for secondary mineralization of Haversian bone using synchrotron Fourier transform infrared micro spectroscopy
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DOI：
10.1016/j.matbio.2007.07.006
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发表时间：
2008-01-01
期刊：
影响因子：
6.9
通讯作者：
Burr, David B.
中科院分区：
文献类型：
--
作者：
Fuchs, Robyn K.;Allen, Matt R.;Burr, David B.
在组织水平上，已经确定重塑速率与矿化程度有关。然而，目前尚不清楚在二次矿化过程中，单个骨结构单位（BSU）需要多长时间才能完全矿化。利用同步辐射傅里叶变换红外显微光谱（FTIRM），我们研究了新形成的骨基质达到生理矿化极限所需的时间。在特定时间点，对26只4月龄雌性新西兰白色家兔给予多达4种不同的荧光染料标记物，以评价胫骨骨干中矿化1、8、18、35、70、105、140、175、210、245、280、315、350和385天的标记骨单位的化学组成。来自505日龄兔的间质骨用作骨矿化的生理极限的参考值。使用同步加速器FTIRM，对蛋白质（酰胺I：1688-1623 cm（-1））、碳酸盐（v（2）CO（3）（2-）：905-825 cm（-1））和磷酸盐（v（4）PO（4）（3-）：650-500 cm（-1））IR谱带进行面积积分。IR光谱数据表示为磷酸盐/蛋白质（总基质矿化）和碳酸盐/蛋白质的比值。骨细胞骨的矿化率在第1天至第18天之间迅速进行，达到间质骨水平的67%。随后是更缓慢、更渐进的矿物质积累，直至第350天。到了350天，增长率趋于稳定。碳酸盐/蛋白质的比率在前18天也迅速增加，达到间质骨水平的73%。碳酸盐/蛋白质的比率在第315天达到稳定，达到与间质骨水平无显著差异的水平。总之，我们的数据表明，骨在前18天内迅速积累矿物质（初级矿化），随后是矿物质积累的逐渐增加（次级矿化），我们发现在350天内完成。(C)2007年Elsevier B. V./国际矩阵生物学学会。All rights reserved.
At the tissue level it is well established that the rate of remodeling is related to the degree of mineralization. However, it is unknown how long it takes for an individual bone structural unit (BSU) to become fully mineralized during secondary mineralization. Using synchrotron Fourier transform infrared microspectroscopy (FTIRM) we examined the time required for newly formed bone matrix to reach a physiological mineralization limit. Twenty-six, four-month old female New Zealand white rabbits were administered up to four different fluorochrome labels at specific time points to evaluate the chemical composition of labeled osteons from the tibial diaphysis that had mineralized for 1, 8, 18, 35, 70, 105, 140, 175, 210, 245, 280, 315, 350, and 385 days. Interstitial bone from 505 day old rabbits was used as a reference value for the physiological limit to which bone mineralizes. Using synchrotron FTIRM, area integrations were carried out on protein (Amide I: 1688-1623 cm(-1)), carbonate (v(2)CO(3)(2-): 905-825 cm(-1)), and phosphate (v(4)PO(4)(3-): 650-500 cm(-1)) IR bands. IR spectral data are presented as ratios of phosphate/protein (overall matrix mineralization) and carbonate/protein. The rate of mineralization of osteonal bone proceeded rapidly between day I and 18, reaching 67% of interstitial bone levels. This was followed by a slower, more progressive accumulation of mineral up to day 350. By 350 days the rate of increase plateaued. The ratio of carbonate/protein also increased rapidly during the first 18 days, reaching 73% of interstitial bone levels. The ratio of carbonate/protein plateaued by day 315, reaching levels not significantly different to interstitial bone levels. In conclusion, our data demonstrate that bone accumulates mineral rapidly during the first 18 days (primary mineralization), followed by a more gradual increase in the accumulation of mineral (secondary mineralization) which we found to be completed in 350 days. (C) 2007 Elsevier B.V./International Society of Matrix Biology. All rights reserved.