Phylogenetic analysis and arsenate reduction effect of the arsenic-reducing bacteria enriched from contaminated soils at an abandoned smelter site.
Phylogenetic analysis and arsenate reduction effect of the arsenic-reducing bacteria enriched from contaminated soils at an abandoned smelter site.
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废弃冶炼厂污染土壤富集砷还原菌的系统发育分析及砷酸还原效果。
DOI：
10.1016/s1001-0742(08)62556-5
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发表时间：
2008
影响因子：
6.9
通讯作者：
中科院分区：
文献类型：
--
作者：
As（V）的微生物还原（即，砷酸盐对水环境中砷的迁移起着重要作用。本研究以某冶炼厂废弃地砷污染土壤中富集的细菌为研究对象，研究其还原砷的特性。发现As（V）完全还原为As（III）（即，亚砷酸盐）。孵育3 d后，沉淀出黄色固体，As（III）浓度急剧下降。孵育150 h后，约。65%的可溶性砷被从溶液中去除。通过扫描电镜、能谱仪（SEM-EDS）和X射线衍射仪（XRD）对沉淀物进行分析，结果表明沉淀物的主要成分为晶态硫化砷（AsS）。还研究了微生物介导的氢氧化铁上吸附的As（V）的还原和移动。在微观浆料实验中，ca。53%的吸附态As（V）被细菌还原为As（III），从而使砷释放到水相中。释放的砷主要以As（III）的形式存在。采用16 SrDNA分子遗传学方法对微生物多样性进行了分析。构建了近全长16 S rDNA基因克隆文库。利用限制性片段长度多态性（RFLP）技术对197个克隆进行分析，得到72个OTU，占6个OTU中克隆总数的51%。选择这六个OTU中的六个细菌克隆进行测序，发现测序的克隆属于Caloramator、梭菌属和芽孢杆菌属。
Microbial reduction of As(V) (i.e., arsenate) plays an important role in arsenic (As) mobilization in aqueous environment. In this study, we investigated As(V) reduction characteristics of the bacteria enriched from the arsenic-contaminated soil at an abandoned smelter site. It was found that As(V) was completely reduced to As(III) (i.e., arsenite) in 21 h. After 3-d incubation, a yellow solid was precipitated and the concentration of As(III) decreased sharply. After 150 h incubation, ca. 65% of soluble arsenic was removed from the solution. The analysis of the precipitate by scanning electron microscopy and energy dispersive spectrometer (SEM-EDS) and X-ray diffraction (XRD) revealed that the main component was crystalline arsenic sulfide (AsS). Microbial mediated reduction and mobilization of adsorbed As(V) on ferric hydroxide was also examined. In the microcosm slurry experiment, ca. 53% of the adsorbed As(V) was reduced to As(III) by the bacteria, which resulted in an appreciable release of arsenic into aqueous phase. The released arsenic was present predominantly as As(III). The microbial diversity was analyzed by 16S rDNA-dependent molecular phylogeny. A near-full-length 16S rDNA gene clone library was constructed. The 197 clones were analyzed using RFLP (restriction fragment length polymorphism) and 72 OTUs were obtained, which contributed 51% of the content for total clone number in six OTUs. Six bacterial clones in these six OTUs were selected for sequencing and the sequenced clones were found to belong to the group Caloramator, Clostridium, and Bacillus.
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影响因子：
11.4
作者：
Ahmann, D;Krumholz, LR;Morel, FMM
通讯作者：
Morel, FMM
影响因子：
1.4
作者：
S. Quináia;M. Rollemberg
通讯作者：
S. Quináia;M. Rollemberg
影响因子：
11.4
作者：
Raven, KP;Jain, A;Loeppert, RH
通讯作者：
Loeppert, RH
影响因子：
64.8
作者：
Islam, FS;Gault, AG;Lloyd, JR
通讯作者：
Lloyd, JR
影响因子：
2.3
作者：
J. Zachara;R. Kukkadapu;J. Fredrickson;Y. Gorby;Steven C. Smith
通讯作者：
J. Zachara;R. Kukkadapu;J. Fredrickson;Y. Gorby;Steven C. Smith